TY - JOUR
T1 - Whole embryo culture, transcriptomics and RNA interference identify TBX1 and FGF11 as novel regulators of limb development in the mouse
AU - Tejedor, Gautier
AU - Laplace-Builhé, Béryl
AU - Luz-Crawford, Patricia
AU - Assou, Said
AU - Barthelaix, Audrey
AU - Mathieu, Marc
AU - Kissa, Karima
AU - Jorgensen, Christian
AU - Collignon, Jérôme
AU - Chuchana, Paul
AU - Djouad, Farida
N1 - Funding Information:
We thank the MRI facilities for their assistance and SMARTY platform and Network of Animal facilities of Montpellier. This work was supported by Inserm, the University of Montpellier and grants from the Région Languedoc-Roussillon Chercheur(se)s d’Avenir. It contributes also to the Labex "Who am I?" #ANR-11-LABX-0071 and the Université de Paris Idex #ANR-18-IDEX-0001 funded by the french government.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Identifying genes involved in vertebrate developmental processes and characterizing this involvement are daunting tasks, especially in the mouse where viviparity complicates investigations. Attempting to devise a streamlined approach for this type of study we focused on limb development. We cultured E10.5 and E12.5 embryos and performed transcriptional profiling to track molecular changes in the forelimb bud over a 6-hour time-window. The expression of certain genes was found to diverge rapidly from its normal path, possibly reflecting the activation of a stress-induced response. Others, however, maintained for up to 3 hours dynamic expression profiles similar to those seen in utero. Some of these resilient genes were known regulators of limb development. The implication of the others in this process was either unsuspected or unsubstantiated. The localized knockdown of two such genes, Fgf11 and Tbx1, hampered forelimb bud development, providing evidence of their implication. These results show that combining embryo culture, transcriptome analysis and RNA interference could speed up the identification of genes involved in a variety of developmental processes, and the validation of their implication.
AB - Identifying genes involved in vertebrate developmental processes and characterizing this involvement are daunting tasks, especially in the mouse where viviparity complicates investigations. Attempting to devise a streamlined approach for this type of study we focused on limb development. We cultured E10.5 and E12.5 embryos and performed transcriptional profiling to track molecular changes in the forelimb bud over a 6-hour time-window. The expression of certain genes was found to diverge rapidly from its normal path, possibly reflecting the activation of a stress-induced response. Others, however, maintained for up to 3 hours dynamic expression profiles similar to those seen in utero. Some of these resilient genes were known regulators of limb development. The implication of the others in this process was either unsuspected or unsubstantiated. The localized knockdown of two such genes, Fgf11 and Tbx1, hampered forelimb bud development, providing evidence of their implication. These results show that combining embryo culture, transcriptome analysis and RNA interference could speed up the identification of genes involved in a variety of developmental processes, and the validation of their implication.
UR - http://www.scopus.com/inward/record.url?scp=85081001808&partnerID=8YFLogxK
U2 - 10.1038/s41598-020-60217-w
DO - 10.1038/s41598-020-60217-w
M3 - Article
C2 - 32107392
AN - SCOPUS:85081001808
SN - 2045-2322
VL - 10
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 3597
ER -