TY - JOUR
T1 - Use of Human Umbilical Cord and Its Byproducts in Tissue Regeneration
AU - Velarde, Francesca
AU - Castañeda, Verónica
AU - Morales, Emilia
AU - Ortega, Mayra
AU - Ocaña, Edwin
AU - Álvarez-Barreto, Jose
AU - Grunauer, Michelle
AU - Eguiguren, Luis
AU - Caicedo, Andrés
N1 - Funding Information:
We are grateful to the health professionals of Hospital Carlos Andrade Mar?n and to Drs. A. Zurita and S. Nicola for the care provided to the patients. AC would like to thank Sistemas Medicos-USFQ (SIME-USFQ) for their support. Funding. This research was funded by the USFQ School of Medicine Grant (institutional code: HUBi 5502). The USFQ evaluated this project before its submission to Frontiers through grant proposals and bioethics review processes according to institutional criteria. Funding bodies did not play any role in the design, data collection, analysis, and interpretations of data or in writing the manuscript.
Publisher Copyright:
© Copyright © 2020 Velarde, Castañeda, Morales, Ortega, Ocaña, Álvarez-Barreto, Grunauer, Eguiguren and Caicedo.
PY - 2020/3/10
Y1 - 2020/3/10
N2 - The fresh or cryopreserved human umbilical cord (HUC) and its byproducts, such as cells and extracts, have different uses in tissue regeneration. Defining what HUC byproduct is more effective in a particular application is a challenge. Furthermore, the methods of isolation, culture and preservation, may affect cell viability and regenerative properties. In this article, we review the HUC and its byproducts’ applications in research and clinical practice. We present our results of successful use of HUC as a patch to treat gastroschisis and its potential to be applied in other conditions. Our in vitro results show an increase in proliferation and migration of human fibroblasts by using an acellular HUC extract. Our goal is to promote standardization of procedures and point out that applications of HUC and its byproducts, as well as the resulting advances in regenerative medicine, will depend on rigorous quality control and on more research in this area.
AB - The fresh or cryopreserved human umbilical cord (HUC) and its byproducts, such as cells and extracts, have different uses in tissue regeneration. Defining what HUC byproduct is more effective in a particular application is a challenge. Furthermore, the methods of isolation, culture and preservation, may affect cell viability and regenerative properties. In this article, we review the HUC and its byproducts’ applications in research and clinical practice. We present our results of successful use of HUC as a patch to treat gastroschisis and its potential to be applied in other conditions. Our in vitro results show an increase in proliferation and migration of human fibroblasts by using an acellular HUC extract. Our goal is to promote standardization of procedures and point out that applications of HUC and its byproducts, as well as the resulting advances in regenerative medicine, will depend on rigorous quality control and on more research in this area.
KW - cryopreserved allograft
KW - gastroschisis
KW - mesenchymal stem cells
KW - regenerative medicine
KW - tissue regeneration
KW - umbilical cord
KW - umbilical cord extracts
KW - umbilical cord stem cells
UR - http://www.scopus.com/inward/record.url?scp=85082691209&partnerID=8YFLogxK
U2 - 10.3389/fbioe.2020.00117
DO - 10.3389/fbioe.2020.00117
M3 - Article
AN - SCOPUS:85082691209
SN - 2296-4185
VL - 8
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
M1 - 117
ER -
