Two-dimensional gel electrophoresis-based proteomic analysis of brain synapses

Karl Heinz Smalla*, Úrsula Wyneken

*Autor correspondiente de este trabajo

Resultado de la investigación: Capítulo del libro/informe/acta de congresoCapítulorevisión exhaustiva

1 Cita (Scopus)

Resumen

Neuroproteomic technologies are crucial for a deeper understanding of molecular mechanisms underlying neuronal plasticity and pathologies of the central nervous system. For a comprehensive neuroproteomic analysis, high-resolution high-throughput techniques are indispensable. The most commonly used-system for 2D gel electrophoresis is based on the combination of isoelectric focussing and sodium dodecyl-sulphate-polyacrylamide gel electrophoresis. Nevertheless, the analysis of complex samples derived from biochemically prepared synaptic fractions raises some crucial challenges. Therefore, we describe the-general 2D gel electrophoresis procedure, including sample preparation, gel casting, electrophoresis-condition, and the detection of proteins on the gel with mass spectrometry compatible silver staining, sypro-ruby or colloidal Coomassie Brilliant Blue.

Idioma originalInglés
Título de la publicación alojadaNeuroproteomics
EditoresKa Wan Li
Páginas95-113
Número de páginas19
DOI
EstadoPublicada - 2011
Publicado de forma externa

Serie de la publicación

NombreNeuromethods
Volumen57
ISSN (versión impresa)0893-2336
ISSN (versión digital)1940-6045

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