TY - JOUR
T1 - Synthesis of nanostructured porous silica coatings on titanium and their cell adhesive and osteogenic differentiation properties
AU - Inzunza, Débora
AU - Covarrubias, Cristian
AU - Marttens, Alfredo Von
AU - Leighton, Yerko
AU - Carvajal, Juan Carlos
AU - Valenzuela, Francisco
AU - Díaz-Dosque, Mario
AU - Méndez, Nicolás
AU - Martínez, Constanza
AU - Pino, Ana María
AU - Rodríguez, Juan Pablo
AU - Cáceres, Mónica
AU - Smith, Patricio
N1 - © 2013 Wiley Periodicals, Inc.
PY - 2014/1
Y1 - 2014/1
N2 - Nanostructured porous silica coatings were synthesized on titanium by the combined sol-gel and evaporation-induced self-assembly process. The silica-coating structures were characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and nitrogen sorptometry. The effect of the nanoporous surface on apatite formation in simulated body fluid, protein adsorption, osteoblast cell adhesion behavior, and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) is reported. Silica coatings with highly ordered sub-10 nm porosity accelerate early osteoblast adhesive response, a favorable cell response that is attributed to an indirect effect due to the high protein adsorption observed on the large-specific surface area of the nanoporous coating but is also probably due to direct mechanical stimulus from the nanostructured topography. The nanoporous silica coatings, particularly those doped with calcium and phosphate, also promote the osteogenic differentiation of hBMSCs with spontaneous mineral nodule formation in basal conditions. The bioactive surface properties exhibited by the nanostructured porous silica coatings make these materials a promising alternative to improve the osseointegration properties of titanium dental implants and could have future impact on the nanoscale design of implant surfaces.
AB - Nanostructured porous silica coatings were synthesized on titanium by the combined sol-gel and evaporation-induced self-assembly process. The silica-coating structures were characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and nitrogen sorptometry. The effect of the nanoporous surface on apatite formation in simulated body fluid, protein adsorption, osteoblast cell adhesion behavior, and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) is reported. Silica coatings with highly ordered sub-10 nm porosity accelerate early osteoblast adhesive response, a favorable cell response that is attributed to an indirect effect due to the high protein adsorption observed on the large-specific surface area of the nanoporous coating but is also probably due to direct mechanical stimulus from the nanostructured topography. The nanoporous silica coatings, particularly those doped with calcium and phosphate, also promote the osteogenic differentiation of hBMSCs with spontaneous mineral nodule formation in basal conditions. The bioactive surface properties exhibited by the nanostructured porous silica coatings make these materials a promising alternative to improve the osseointegration properties of titanium dental implants and could have future impact on the nanoscale design of implant surfaces.
KW - cell adhesion
KW - nanotopography
KW - osseointegration
KW - sol-gel technique
KW - titanium
UR - http://www.scopus.com/inward/record.url?scp=84888645926&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.34673
DO - 10.1002/jbm.a.34673
M3 - Article
C2 - 23568757
AN - SCOPUS:84888645926
SN - 1549-3296
VL - 102
SP - 37
EP - 48
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 1
ER -