Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β -Cells Treated with Palmitate and Oleate

L. R. Cataldo, M. L. Mizgier, D. Busso, P. Olmos, J. E. Galgani, R. Valenzuela, D. Mezzano, E. Aranda, V. A. Cortés, J. L. Santos*

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

7 Citas (Scopus)

Resumen

High circulating nonesterified fatty acids (NEFAs) concentration, often reported in diabetes, leads to impaired glucose-stimulated insulin secretion (GSIS) through not yet well-defined mechanisms. Serotonin and dopamine might contribute to NEFA-dependent β-cell dysfunction, since extracellular signal of these monoamines decreases GSIS. Moreover, palmitate-treated β-cells may enhance the expression of the serotonin receptor Htr2c, affecting insulin secretion. Additionally, the expression of monoamine-oxidase type B (Maob) seems to be lower in islets from humans and mice with diabetes compared to nondiabetic islets, which may lead to increased monoamine concentrations. We assessed the expression of serotonin- and dopamine-related genes in islets from db/db and wild-type (WT) mice. In addition, the effect of palmitate and oleate on the expression of such genes, 5HT content, and GSIS in MIN6 β-cell was determined. Lower Maob expression was found in islets from db/db versus WT mice and in MIN6 β-cells in response to palmitate and oleate treatment compared to vehicle. Reduced 5HT content and impaired GSIS in response to palmitate (-25%; p < 0.0001) and oleate (-43%; p < 0.0001) were detected in MIN6 β-cells. In conclusion, known defects of GSIS in islets from db/db mice and MIN6 β-cells treated with NEFAs are accompanied by reduced Maob expression and reduced 5HT content.

Idioma originalInglés
Número de artículo3793781
PublicaciónJournal of Diabetes Research
Volumen2016
DOI
EstadoPublicada - 2016
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
© 2016 L. R. Cataldo et al.

Huella

Profundice en los temas de investigación de 'Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 β -Cells Treated with Palmitate and Oleate'. En conjunto forman una huella única.

Citar esto