Oral polio vaccine in infants does not interfere in detection of enterovirus in blood

Título traducido de la contribución: La vacuna polio oral en lactantes no interfiere con la detección de enterovirus en sangre

Marcela González, Carmen Sandoval, Patricia Valenzuela, Luisa Montecinos, Constanza Martínez, Paula Godoy, Katia Abarca*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Introduction: There is not known if a viraemia post-oral polio vaccine (OPV) is detectable by modern molecular techniques. Such viraemia could affect the performance of the real time-polymerase chain reaction (PCR) for non polio enterovirus (EV) detection, technique of growing clinical use for the study of febrile infants. Objective: To determine viraemia post-first dose of OPV in healthy infants, by molecular techniques. Patients and Methods: 50 infants less than three months without previous VPO were randomized in 5 groups: a control group with prevaccination blood sample (BS), group 1 BS at day 2, group 2 BS at day 4, group 3, BS at day 6 and group 4, BS at day 8 post-vaccination. Conventional and specific PCR for poliovirus and real time PCR for non polio EV were performed in BS and in OPV samples. Results: No genetic material of poliovirus was detected in any infant, while in 9 of them (18%) non polio EV was identified. Real time PCR for EV did not amplify poliovirus from OPV samples. Discussion: Results suggest that no post VPO viraemia detectable by molecular methods exists. Considering that real time PCR for EV does not allow to identify polio virus, no false positives of the test are expected as a result of a recent VPO vaccination. We documented presence of non polio EV in blood of healthy asymptomatic infants.

Título traducido de la contribuciónLa vacuna polio oral en lactantes no interfiere con la detección de enterovirus en sangre
Idioma originalInglés
Páginas (desde-hasta)626-629
Número de páginas4
PublicaciónRevista Chilena de Infectologia
Volumen30
N.º6
DOI
EstadoPublicada - dic. 2013
Publicado de forma externa

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