One-step combined focused epPCR and saturation mutagenesis for thermostability evolution of a new cold-active xylanase

Ingeniería y ciencia de los materiales

• Bacteria 10%
• Biocatalysis 16%
• Computer simulation 4%
• Costs 3%
• Enzymes 72%
• Escherichia coli 11%
• Industry 3%
• Molecular dynamics 10%
• Mutagenesis 100%
• Proteins 16%
• Stereoselectivity 18%
• Temperature 7%
• Thermodynamic properties 8%
• Thermodynamic stability 64%

Medicina y ciencias biológicas

• Antarctic Regions 13%
• Bacteria 6%
• Biocatalysis 15%
• Catalytic Domain 8%
• Clone Cells 7%
• Cold Temperature 56%
• Costs and Cost Analysis 5%
• Enzymes 34%
• Escherichia coli 6%
• Industry 8%
• Libraries 7%
• Molecular Dynamics Simulation 10%
• Mutagenesis 62%
• Mutation 5%
• Protein Engineering 12%
• Proteins 3%
• Psychrobacter 16%
• Temperature 12%

Compuestos químicos

• Application 9%
• Energy 14%
• Enzyme Activity 25%
• Industry 23%
• Molecular Dynamics 23%
• Mutation 26%
• Protein 26%
• Simulation 19%
• Stereoselectivity 29%
• Strain 43%