NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration

Béryl Laplace-Builhé, Audrey Barthelaix, Said Assou, Candice Bohaud, Marine Pratlong, Dany Severac, Gautier Tejedor, Patricia Luz-Crawford, Mai Nguyen-Chi, Marc Mathieu, Christian Jorgensen, Farida Djouad*

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

3 Citas (Scopus)

Resumen

Fish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.
Idioma originalInglés
Número de artículo6336
Páginas (desde-hasta)6336
PublicaciónNature Communications
Volumen12
N.º1
DOI
EstadoPublicada - 3 nov. 2021

Nota bibliográfica

Publisher Copyright:
© 2021, The Author(s).

Palabras clave

  • Animal Fins
  • Animals
  • Cell Proliferation
  • Forkhead Transcription Factors
  • Gene Expression Regulation, Neoplastic
  • Developmental; Genes
  • erbB
  • Larva
  • Macrophages
  • Neural Crest
  • Neuregulin-1
  • Regeneration
  • Signal Transduction
  • Stem Cells
  • Zebrafish
  • Zebrafish Proteins

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