Molecular signatures of extracellular vesicles in oral fluids of periodontitis patients

Alejandra Chaparro Padilla*, Laura Weber Aracena, Ornella Realini Fuentes, Daniela Albers Busquetts, Marcela Hernández Ríos, Valeria Ramírez Lobos, Andrés Pascual La Rocca, José Nart Molina, Victor Beltrán Varas, Stephanie Acuña-Gallardo, Antonio Sanz Ruiz

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

Objective
To characterize extracellular vesicles (EVs) in gingival crevicular fluid (GCF) and saliva samples from healthy/gingivitis and periodontitis patients and correlate them with clinical inflammatory periodontal parameters.

Material and Method
An exploratory study, including 86 subjects, was conducted. Clinical and periodontal data were recorded, and oral fluid samples were obtained. EVs were precipitated by ExoQuick-TC™ and characterized by nanoparticle tracking (NanoSight™), Western blot (WB), transmission electron microscopy (TEM), and ELISA analysis.

Results
TEM showed nanoparticles morphologically compatible with EVs, and WB analysis revealed bands of specific EV markers (CD9, TSG101, and Alix) in both oral fluids of periodontitis and healthy/gingivitis subjects. The total concentration of EVs in GCF was increased in periodontitis patients compared to healthy/gingivitis subjects (p = .017). However, we did not observe differences in the EV concentration of saliva samples (p = .190). The size of GCF-EVs was 144.2 nm in periodontitis and 160.35 nm in healthy/gingivitis patients (p = .038). The CD63 exosome marker was increased in GCF of periodontitis patients (p = .00001). The total concentration of EVs in GCF was correlated with bleeding on probing (rho = 0.63, p = .002), periodontal probing depth (rho = 0.56, p = .009), and clinical attachment level (rho = 0.48, p = .030).

Conclusion
Periodontitis patients have an increased concentration of EVs in GCF, and their role in periodontitis should be clarified.
Idioma originalInglés
Páginas (desde-hasta)1318-1325
Número de páginas8
PublicaciónOral Diseases
Volumen26
N.º6
DOI
EstadoPublicada - 1 sep 2020

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© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved

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