Mitochondrial MicroRNAs Contribute to Macrophage Immune Functions Including Differentiation, Polarization, and Activation

Isabelle Duroux-Richard, Florence Apparailly*, Maroun Khoury

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

1 Cita (Scopus)

Resumen

A subset of microRNA (miRNA) has been shown to play an important role in mitochondrial (mt) functions and are named MitomiR. They are present within or associated with mitochondria. Most of the mitochondrial miRNAs originate from the nucleus, while a very limited number is encoded by mtDNA. Moreover, the miRNA machinery including the Dicer and Argonaute has also been detected within mitochondria. Recent, literature has established a close relationship between miRNAs and inflammation. Indeed, specific miRNA signatures are associated with macrophage differentiation, polarization and functions. Nevertheless, the regulation of macrophage inflammatory pathways governed specifically by MitomiR and their implication in immune-mediated inflammatory disorders remain poorly studied. Here, we propose a hypothesis in which MitomiR play a key role in triggering macrophage differentiation and modulating their downstream activation and immune functions. We sustain this proposition by bioinformatic data obtained from either the human monocytic THP1 cell line or the purified mitochondrial fraction of PMA-induced human macrophages. Interestingly, 22% of the 754 assayed miRNAs were detected in the mitochondrial fraction and are either exclusively or highly enriched cellular miRNA. Furthermore, the in silico analysis performed in this study, identified a specific MitomiR signature associated with macrophage differentiation that was correlated with gene targets within the mitochondria genome or with mitochondrial pathways. Overall, our hypothesis and data suggest a previously unrecognized link between MitomiR and macrophage function and fate. We also suggest that the MitomiR-dependent control could be further enhanced through the transfer of mitochondria from donor to target cells, as a new strategy for MitomiR delivery.

Idioma originalInglés
Número de artículo738140
Páginas (desde-hasta)738140
PublicaciónFrontiers in Physiology
Volumen12
DOI
EstadoPublicada - 3 nov. 2021

Nota bibliográfica

Funding Information:
This work was supported by grants from the Chilean National Agency for Investigation and Development: ANID (Agencia Nacional de Investigación y Desarrollo) (FONDECYT regular, 2021, #1211749). In addition to research funds from the INSERM (Institut National de la Santé et Recherche Médicale); the University of Montpellier; and the International research project INSERM France-Chile 2020.

Funding Information:
We thank Francesca Velarde from the University of Los Andes, Chile for the critical proofreading of the manuscript.

Publisher Copyright:
Copyright © 2021 Duroux-Richard, Apparailly and Khoury.

Palabras clave

  • inflammmation
  • macrophage
  • microRNA
  • miRNA
  • mitochondria
  • mitochondria transfer
  • MitomiR

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