Method for measurement of collagen monomer orientation in fluorescence microscopy

Rodrigo P. Alzola, José Francisco Delpiano, Anuj Gajjar, Rickard Stureborg, Seyed Mohammad Siadat, Jeffrey W. Ruberti, Charles A. Dimarzio

Resultado de la investigación: Capítulo del libro/informe/acta de congresoCapítulorevisión exhaustiva

Resumen

Collagen is one of the most important proteins in mammals, conforming most animal tissues. This work explores how a basic collagen monomer unit is visualized using fluorescence microscopy and how its spatial orientation is determined. Defining the orientation of collagen monomers is not a trivial problem, as the particle has a weak contrast and is relatively small. Possible attach fluorescence tags for contrast, but the size is still a problem for detecting orientation using fluorescence microscopy. This document presents a simulation of the visualization of collagen monomers and two methods for detecting monomer and classifying its orientation. A modify Gabor filter set, and an automatic classifier, trained by convolutional neuronal network (CNN), were used. By evaluating the performance of these two approaches compare to human observation, our results show that it is possible to determine the location and orientation of a single monomer with fluorescence microscopy. These findings can contribute to understanding collagen elements as collagen fibril.

Idioma originalInglés
Título de la publicación alojadaThree-Dimensional and Multidimensional Microscopy
Subtítulo de la publicación alojadaImage Acquisition and Processing XXVIII
EditoresThomas G. Brown, Tony Wilson, Laura Waller
EditorialSPIE
Páginas19
ISBN (versión digital)9781510641334
ISBN (versión impresa)9781510641334
DOI
EstadoPublicada - 4 mar 2021
EventoThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII 2021 - Virtual, Online, Estados Unidos
Duración: 6 mar 202111 mar 2021

Serie de la publicación

NombreProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volumen11649
ISSN (versión impresa)1605-7422

Conferencia

ConferenciaThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII 2021
País/TerritorioEstados Unidos
CiudadVirtual, Online
Período6/03/2111/03/21

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