Método de Recuperación para Piezas Anatómicas del Sistema Nervioso Central

Marcos Valenzuela Oróstica; Reinaldo Soto

doi:10.4067/S0717-95022014000200032

Método de Recuperación para Piezas Anatómicas del Sistema Nervioso Central

Marcos Valenzuela Oróstica^*, Reinaldo Soto

^*Autor correspondiente de este trabajo

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

Resumen

El material anatómico del sistema nervioso central es cada vez más difícil de obtener. A pesar de estar fijado, es muy lábil y al ser expuesto a diversas condiciones ambientales durante las actividades docentes, se va deteriorando, resecándose, adquiriendo una consistencia rígida y un aspecto oscuro que finalmente hace difícil el reconocimiento de estructuras. De la misma manera, cuando obtenemos una pieza de un cadáver con larga data de fallecido, nos podemos encontrar con un encéfalo que por lo reseco de su estado, no presta mayor utilidad. El objetivo de esta técnica es recuperar estas muestras, para que puedan ser utilizadas convenientemente en el estudio anatómico. Se usaron distintos segmentos de encéfalo, incluidos algunos contaminados por hongos y otros obtenidos de cadáveres antiguos. Los materiales utilizados fueron, agua oxigenada, agua destilada, formalina y recipientes plásticos. Se comienza limpiando manualmente las muestras de restos de polvo y cuerpos extraños que se encuentren en su superficie. Se continúa con baños en agua oxigenada, intercalando con rehidrataciones en agua destilada, hasta obtener el color y textura deseados que permitan distinguir macroscópicamente estructuras de la muestra. Posteriormente se refuerza la fijación sumergiéndolas en formalina, para luego conservarlas en forma indefinida, humedecidas con este fijador en bolsas plásticas selladas y dentro de caja plásticas tapadas. Otras muestras fueron plastinadas posteriormente. Al finalizar la técnica la mayoría de las muestras se recuperaron notoriamente, permitiendo reconocer estructuras que por su deterioro era imposible apreciar con anterioridad. En conclusión, este método permite recuperar y darle uso a muestras que estaban prácticamente desechadas.

Título traducido de la contribución	Recovery method for anatomical pieces from the central nervous system
Idioma original	Español
Páginas (desde-hasta)	571-574
Número de páginas	4
Publicación	International Journal of Morphology
Volumen	32
N.º	2
DOI	https://doi.org/10.4067/S0717-95022014000200032
Estado	Publicada - jun. 2014

Acceder al documento

10.4067/S0717-95022014000200032

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@article{6188edb7e6754f6fac3600a00dea31be,

title = "M{\'e}todo de Recuperaci{\'o}n para Piezas Anat{\'o}micas del Sistema Nervioso Central",

abstract = "The anatomical material of the central nervous system is increasingly difficult to obtain. Despite being fixed, is very labile and when exposed to various environmental conditions during teaching, becomes deteriorated and drier, acquiring a rigid consistency and darker appearance, which eventually makes it difficult to recognize structures. At the same time, when we obtain a piece of a long-standing cadaver, we can come across a brain so dry, that its state does not provide any use. The aim of this technique was to recover these samples so they can be conveniently used for anatomical studies. We used various brain segments, including some contaminated with fungi and others obtained from old cadavers. The materials used were, hydrogen peroxide, distilled water, formaldehyde and plastic containers. We begin by manually cleaning the samples from any dust and foreign bodies that could be found on their surface. Then, continues with hydrogen peroxide baths, interspersing with re-hydration in distilled water, until we obtain the desired color and texture that allows us to macroscopically distinguish the structures. Subsequently, we reinforce fixation by immersing in formaldehyde. Subsequently, we keep them moistened with this fixation agent in sealed plastic bags indefinitely, inside covered plastic boxes. Other samples were subsequently plastinated. After we applied this technique, most of the samples were noticeably recovered, allowing recognition of structures that, previously because of their deterioration, were impossible to see. In conclusion, this method allows the recovering and gives use to samples that had been previously discarded.",

keywords = "Central nervous system samples, Hydrogen peroxide, Re-hydration, Recovery",

author = "{Valenzuela Or{\'o}stica}, Marcos and Reinaldo Soto",

year = "2014",

month = jun,

doi = "10.4067/S0717-95022014000200032",

language = "Spanish",

volume = "32",

pages = "571--574",

journal = "International Journal of Morphology",

issn = "0717-9367",

publisher = "Universidad de la Frontera",

number = "2",

}

TY - JOUR

T1 - Método de Recuperación para Piezas Anatómicas del Sistema Nervioso Central

AU - Valenzuela Oróstica, Marcos

AU - Soto , Reinaldo

PY - 2014/6

Y1 - 2014/6

N2 - The anatomical material of the central nervous system is increasingly difficult to obtain. Despite being fixed, is very labile and when exposed to various environmental conditions during teaching, becomes deteriorated and drier, acquiring a rigid consistency and darker appearance, which eventually makes it difficult to recognize structures. At the same time, when we obtain a piece of a long-standing cadaver, we can come across a brain so dry, that its state does not provide any use. The aim of this technique was to recover these samples so they can be conveniently used for anatomical studies. We used various brain segments, including some contaminated with fungi and others obtained from old cadavers. The materials used were, hydrogen peroxide, distilled water, formaldehyde and plastic containers. We begin by manually cleaning the samples from any dust and foreign bodies that could be found on their surface. Then, continues with hydrogen peroxide baths, interspersing with re-hydration in distilled water, until we obtain the desired color and texture that allows us to macroscopically distinguish the structures. Subsequently, we reinforce fixation by immersing in formaldehyde. Subsequently, we keep them moistened with this fixation agent in sealed plastic bags indefinitely, inside covered plastic boxes. Other samples were subsequently plastinated. After we applied this technique, most of the samples were noticeably recovered, allowing recognition of structures that, previously because of their deterioration, were impossible to see. In conclusion, this method allows the recovering and gives use to samples that had been previously discarded.

AB - The anatomical material of the central nervous system is increasingly difficult to obtain. Despite being fixed, is very labile and when exposed to various environmental conditions during teaching, becomes deteriorated and drier, acquiring a rigid consistency and darker appearance, which eventually makes it difficult to recognize structures. At the same time, when we obtain a piece of a long-standing cadaver, we can come across a brain so dry, that its state does not provide any use. The aim of this technique was to recover these samples so they can be conveniently used for anatomical studies. We used various brain segments, including some contaminated with fungi and others obtained from old cadavers. The materials used were, hydrogen peroxide, distilled water, formaldehyde and plastic containers. We begin by manually cleaning the samples from any dust and foreign bodies that could be found on their surface. Then, continues with hydrogen peroxide baths, interspersing with re-hydration in distilled water, until we obtain the desired color and texture that allows us to macroscopically distinguish the structures. Subsequently, we reinforce fixation by immersing in formaldehyde. Subsequently, we keep them moistened with this fixation agent in sealed plastic bags indefinitely, inside covered plastic boxes. Other samples were subsequently plastinated. After we applied this technique, most of the samples were noticeably recovered, allowing recognition of structures that, previously because of their deterioration, were impossible to see. In conclusion, this method allows the recovering and gives use to samples that had been previously discarded.

KW - Central nervous system samples

KW - Hydrogen peroxide

KW - Re-hydration

KW - Recovery

UR - http://www.scopus.com/inward/record.url?scp=84904265682&partnerID=8YFLogxK

U2 - 10.4067/S0717-95022014000200032

DO - 10.4067/S0717-95022014000200032

M3 - Article

AN - SCOPUS:84904265682

SN - 0717-9367

VL - 32

SP - 571

EP - 574

JO - International Journal of Morphology

JF - International Journal of Morphology

IS - 2

ER -

Método de Recuperación para Piezas Anatómicas del Sistema Nervioso Central

Resumen

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto