Layer-by-layer approach for a uniformed fabrication of a cell patterned vessel-like construct

Camila A. Wilkens, Christopher J. Rivet, Tamara L. Akentjew, Julian Alverio, Maroun Khoury, Juan Pablo Acevedo*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

29 Citas (Scopus)


Successful tissue engineered small diameter blood vessels (SDBV) require manufacturing systems capable of precisely controlling different key elements, such as material composition, geometry and spatial location of specialized biomaterials and cells types. We report in this work an automated methodology that enables the manufacture of multilayer cylindrical constructs for SDBV fabrication that uses a layer-by-layer deposition approach while controlling variables such as dipping and spinning speed of a rod and biomaterial viscosity. Different biomaterials including methacrylated gelatin, alginate and chitosan were tested using this procedure to build different parts of the constructs. The system was capable of controlling dimensions of lumen from 0.5 mm up to 6 mm diameter and individual layers from 1 μm up to 400 μm thick. A cellular component was successfully added to the biomaterial in the absence of significant cytotoxic effect which was assessed by viability and proliferation assays. Additionally, cells showed a homogenous distribution with well-defined concentric patterns across the multilayer vessel grafts. The challenging generation of inner endothelial cells of approximately 20-30 μm of thickness was achieved. Preliminary experimental evidences of microstructural alignment of the biomaterial were obtained when the dipping approach was combined with the rod rotation. The study demonstrated the wide versatility and scalability of the automated system to easily and rapidly fabricate complex cellularized multilayer vascular grafts with structural configuration that resembles natural blood vessels.
Idioma originalInglés
Número de artículo015001
EstadoPublicada - mar. 2017

Nota bibliográfica

Publisher Copyright:
© 2016 IOP Publishing Ltd.

Palabras clave

  • cell patterning
  • CNC device
  • small diameter blood vessel
  • tissue engineering


Profundice en los temas de investigación de 'Layer-by-layer approach for a uniformed fabrication of a cell patterned vessel-like construct'. En conjunto forman una huella única.

Citar esto