Isolation of the postsynaptic density: a specialization of the subsynaptic cytoskeleton.

Karl Heinz Smalla*, Patricia Klemmer, Ursula Wyneken

*Autor correspondiente de este trabajo

Producción científica: Capítulo del libro/informe/acta de congresoCapítulorevisión exhaustiva

11 Citas (Scopus)


Postsynaptic densities (PSDs) are cytoskeletal specializations present in synaptic contacts in the central nervous system. PSDs have been considered to be subcellular organelles that maintain and cluster the synaptic signal transduction apparatus in direct contact with the actin cytoskeleton and its regulators. Synapses and PSDs are highly dynamic structures that in addition of subserving transmission of information participate in its processing and storage. Therefore, the molecular components of PSDs reveal their signal transduction capacities in health and disease. Here, we present the experimental protocol we have been using for years to isolate PSDs from other cell components, including or excluding detergent-­resistant membranes (or lipid rafts). The protocol can be applied to brain samples of different areas, ages, and mammalian species and is useful to obtain this subcellular organelle in a highly reproducible manner.
Idioma originalInglés
Título de la publicación alojadaThe Cytoskeleton
Subtítulo de la publicación alojadaImaging, Isolation, and Interaction
EditorialHumana Press Inc.
Número de páginas16
ISBN (versión impresa)9781627032650
EstadoPublicada - 2013

Serie de la publicación

ISSN (versión impresa)0893-2336
ISSN (versión digital)1940-6045


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