TY - JOUR
T1 - Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses
AU - Figueroa, Horacio
AU - Lozano, Mauricio
AU - Suazo, Cristian
AU - Eixarch, Elisenda
AU - Illanes, Sebastián
AU - Carreño, Juan Eduardo
AU - Villanueva, Sandra
AU - Hernández-Andrade, Edgar
AU - Gratacós, Eduard
AU - Irarrazabal Muñoz, Carlos Ernesto
N1 - Funding Information:
This research was supported by FAI-MED 003‐09 and FAI‐MED 001‐10 from the University of Los Andes—Chile . FONDECYT-1100885 , FONDECYT-1110869 and FONDECYT-1080373 . E.E. was supported by an Emili Letang fellowship by the Hospital Clinic and a Rio Hortega grant from the Carlos III Institute of Health (Spain) ( CM08/00105 ).
PY - 2012/11
Y1 - 2012/11
N2 - The aim of this work was to study the effect of intrauterine growth restriction (IUGR) on fetal kidneys. The IUGR was induced by uteroplacental vessels ligature in a model of pregnant rabbit. We centralized the study in the gene expression of essential proteins for fetal kidney development and kidney protection against hypoxia, osmotic stress, and kidney injury. The gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM were studied by qRT-PCR and Western blot in kidneys from control and IUGR fetuses. Experimental IUGR fetuses were significantly smaller than the control animals (39 vs. 48. g, p < 0.05). The number of glomeruli was decreased in IUGR kidneys, without morphological alterations. IUGR increased the gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM (p < 0.05) in kidneys of fetuses undergoing IUGR, suggesting that fetal blood flow restriction produce alterations in gene expression in fetal kidneys.
AB - The aim of this work was to study the effect of intrauterine growth restriction (IUGR) on fetal kidneys. The IUGR was induced by uteroplacental vessels ligature in a model of pregnant rabbit. We centralized the study in the gene expression of essential proteins for fetal kidney development and kidney protection against hypoxia, osmotic stress, and kidney injury. The gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM were studied by qRT-PCR and Western blot in kidneys from control and IUGR fetuses. Experimental IUGR fetuses were significantly smaller than the control animals (39 vs. 48. g, p < 0.05). The number of glomeruli was decreased in IUGR kidneys, without morphological alterations. IUGR increased the gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM (p < 0.05) in kidneys of fetuses undergoing IUGR, suggesting that fetal blood flow restriction produce alterations in gene expression in fetal kidneys.
KW - Hypoxia
KW - IUGR
KW - Inflammation
KW - Osmotic stress
KW - Rabbit
UR - http://www.scopus.com/inward/record.url?scp=84866851709&partnerID=8YFLogxK
U2 - 10.1016/j.earlhumdev.2012.07.010
DO - 10.1016/j.earlhumdev.2012.07.010
M3 - Article
C2 - 22944138
AN - SCOPUS:84866851709
SN - 0378-3782
VL - 88
SP - 899
EP - 904
JO - Early Human Development
JF - Early Human Development
IS - 11
ER -