Injectable and release-controlled OP-1 hybrid core-shell nanocapsules enhance distraction osteogenesis in rabbits

Z. S. Haidar, R. C. Hamdy, M. Tabrizian

Resultado de la investigación: Capítulo del libro/informe/acta de congresoContribución a la conferenciarevisión exhaustiva

Resumen

A bone morphogenetic protein-7 (BMP-7, also known as osteogenic protein 1 or OP-1) delivery system for potential de novo bone tissue regeneration and acceleration in distraction osteogenesis (DO) is presented. A liposomal core was incorporated in a shell of alternating layer-by-layer (L-b-L) self-assembled natural polyelectrolytes; alginate and chitosan. Hydrophilic, non-toxic, monodisperse, spherical and stable cationic capsules with an extended shelf-life allowing immediate protein loading prior to clinical administration were formulated. Protein encapsulation and release from the nanocapsules can be modulated by modifying the number of polymer layers assembled on the liposomal core. Controlled, triphasic, linear and sustained release of bioactive OP-1 was observed, demonstrating the core-shell effect, in vitro. In vivo, un-loaded and OP-1 loaded nanocapsules were evaluated in a rabbit model of tibial DO. Regenerate structure analysis followed. Osteogenesis and consolidation were accelerated via a single injection of the core-shell nanocapsules loaded with a dose of no more than 1.0 μg OP-1 accentuating further the role of the hybrid nanocapsules. Hence, a promising and cost-effective carrier for the localized and release-controlled administration of therapeutic growth factors has been formulated.

Idioma originalInglés
Título de la publicación alojada25th Southern Biomedical Engineering Conference 2009
Páginas355-358
Número de páginas4
DOI
EstadoPublicada - 2009
Publicado de forma externa
Evento25th Southern Biomedical Engineering Conference 2009 - Miami, FL, Estados Unidos
Duración: 15 may. 200917 may. 2009

Serie de la publicación

NombreIFMBE Proceedings
Volumen24
ISSN (versión impresa)1680-0737

Conferencia

Conferencia25th Southern Biomedical Engineering Conference 2009
País/TerritorioEstados Unidos
CiudadMiami, FL
Período15/05/0917/05/09

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