Identification of polarized macrophage subsets in zebrafish

Mai Nguyen-Chi, Beryl Laplace-Builhe, Jana Travnickova, Patricia Luz-Crawford, Gautier Tejedor, Quang Tien Phan, Isabelle Duroux-Richard, Jean Pierre Levraud, Karima Kissa, Georges Lutfalla, Christian Jorgensen, Farida Djouad

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

98 Citas (Scopus)

Resumen

While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tumour necrosis factor alpha (tnfa), a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa: eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and Escherichia coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on Fluorescence Activated Cell Sorting (FACS)-sorted tnfa+ and tnfa-macrophages showed that they, respectively, expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa+ macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.
Idioma originalInglés estadounidense
PublicacióneLife
Volumen4
N.ºJULY 2015
DOI
EstadoPublicada - 8 jul 2015
Publicado de forma externa

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