TY - JOUR
T1 - Humidity prevents the exercise-induced formation of hydrogen peroxide and nitrite in exhaled breath condensate in recreational cyclists
AU - Contreras-Briceño, Felipe
AU - Espinosa-Ramirez, Maximiliano
AU - Viscor, Ginés
AU - Araneda, Oscar F.
N1 - Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Purpose: The aerobic exercise affects the respiratory redox-state. The influence of different relative humidity (RH) levels on the formation of respiratory reactive chemical species associated with redox-state altered by exercise has been poorly explored. Our aim was to evaluate the effect of two different RH conditions (40% vs. 90%) on the concentration of hydrogen peroxide and nitrite in exhaled breath condensate ([H2O2]EBC and [NO2−]EBC) and spirometry parameters in recreational cyclists. Methods: Sixteen men and women (12/4) (mean age ± SD: 23.5 ± 2.2 years) completed 60-min of cycling at 166.3 ± 26.9 watts (70% of maximum load of V ˙ O 2-max. test, 49.3 ± 7.6 mL·min−1·kg−1) at random 40%-RH and 90%-RH conditions separated by 7 days. The two-way RM-ANOVA test was applied to compare [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma at rest and 80-min post-exercise (80-post); and spirometry parameters at rest, 20-post and 80-post. Results: The interaction of factors (humidity × time) was significant in [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma (p = 0.005, p = 0.030, p = 0.043, respectively). At 40%-RH conditions, the same parameters were higher in 80-post than at rest (p < 0.001, p = 0.001, p = 0.014, respectively). At the same time, the [H2O2]EBC and [NO2−]EBC/[NO2−]Plasma were higher in 40%-RH than 90%-RH (p = 0.010, p < 0.001, respectively). The interaction was significant in FEV1 (p = 0.013) and FEF25-75% (p = 0.023), but not in FEV1/FVC (p = 0.362). At 80-post, the changes are kept in 90%-RH (p < 0.001), diminishing in 40%-RH being similar to rest. Conclusion: In recreational cyclists, 90%-RH prevents the increase of hydrogen peroxide and nitrite in exhaled breath condensate samples observed at 40%-RH and prolonging the bronchodilation until 80-post cycling exercise.
AB - Purpose: The aerobic exercise affects the respiratory redox-state. The influence of different relative humidity (RH) levels on the formation of respiratory reactive chemical species associated with redox-state altered by exercise has been poorly explored. Our aim was to evaluate the effect of two different RH conditions (40% vs. 90%) on the concentration of hydrogen peroxide and nitrite in exhaled breath condensate ([H2O2]EBC and [NO2−]EBC) and spirometry parameters in recreational cyclists. Methods: Sixteen men and women (12/4) (mean age ± SD: 23.5 ± 2.2 years) completed 60-min of cycling at 166.3 ± 26.9 watts (70% of maximum load of V ˙ O 2-max. test, 49.3 ± 7.6 mL·min−1·kg−1) at random 40%-RH and 90%-RH conditions separated by 7 days. The two-way RM-ANOVA test was applied to compare [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma at rest and 80-min post-exercise (80-post); and spirometry parameters at rest, 20-post and 80-post. Results: The interaction of factors (humidity × time) was significant in [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma (p = 0.005, p = 0.030, p = 0.043, respectively). At 40%-RH conditions, the same parameters were higher in 80-post than at rest (p < 0.001, p = 0.001, p = 0.014, respectively). At the same time, the [H2O2]EBC and [NO2−]EBC/[NO2−]Plasma were higher in 40%-RH than 90%-RH (p = 0.010, p < 0.001, respectively). The interaction was significant in FEV1 (p = 0.013) and FEF25-75% (p = 0.023), but not in FEV1/FVC (p = 0.362). At 80-post, the changes are kept in 90%-RH (p < 0.001), diminishing in 40%-RH being similar to rest. Conclusion: In recreational cyclists, 90%-RH prevents the increase of hydrogen peroxide and nitrite in exhaled breath condensate samples observed at 40%-RH and prolonging the bronchodilation until 80-post cycling exercise.
KW - Bronchodilation
KW - Exercise
KW - Exhaled breath condensate
KW - Humidity
KW - Oxidative stress
KW - Broncodilatación
KW - Ejercicio
KW - Condensado de aliento exhalado
KW - Humedad
KW - Estrés oxidativo
UR - https://www.scopus.com/pages/publications/85089158907
U2 - 10.1007/s00421-020-04456-9
DO - 10.1007/s00421-020-04456-9
M3 - Article
C2 - 32772245
AN - SCOPUS:85089158907
SN - 1439-6319
VL - 120
SP - 2339
EP - 2348
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
IS - 10
ER -
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