Humidity prevents the exercise-induced formation of hydrogen peroxide and nitrite in exhaled breath condensate in recreational cyclists

Felipe Contreras-Briceño; Maximiliano Espinosa-Ramirez; Ginés Viscor; Oscar F. Araneda

doi:10.1007/s00421-020-04456-9

Humidity prevents the exercise-induced formation of hydrogen peroxide and nitrite in exhaled breath condensate in recreational cyclists

Título traducido de la contribución: La humedad previene la formación inducida por el ejercicio de peróxido de hidrógeno y nitrito en el condensado del aire exhalado en ciclistas recreativos

Felipe Contreras-Briceño, Maximiliano Espinosa-Ramirez, Ginés Viscor, Oscar F. Araneda^*

^*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

2 Citas (Scopus)

Resumen

Propósito: El ejercicio aeróbico afecta el estado redox respiratorio. La influencia de diferentes niveles de humedad relativa (HR) en la formación de especies químicas reactivas respiratorias asociadas con el estado redox alterado por el ejercicio ha sido poco explorada. Nuestro objetivo fue evaluar el efecto de dos condiciones de HR diferentes (40% frente a 90%) sobre la concentración de peróxido de hidrógeno y nitrito en el condensado de aire exhalado ([H2O2] EBC y [NO2−] EBC) y parámetros de espirometría en ciclistas recreativos . Métodos: Dieciséis hombres y mujeres (12/4) (edad media ± DE: 23,5 ± 2,2 años) completaron 60 min de ciclismo a 166,3 ± 26,9 vatios (70% de la carga máxima de la prueba V ˙ O 2-máx., 49,3 ± 7,6 mL · min − 1 · kg − 1) en condiciones aleatorias de 40% -RH y 90% -RH separadas por 7 días. La prueba bidireccional RM-ANOVA se aplicó para comparar [H2O2] EBC, [NO2−] EBC, [NO2−] EBC / [NO2−] Plasma en reposo y 80 min post-ejercicio (80-post); y parámetros de espirometría en reposo, 20 postes y 80 postes. Resultados: La interacción de factores (humedad × tiempo) fue significativa en [H2O2] EBC, [NO2−] EBC, [NO2−] EBC / [NO2−] Plasma (p = 0.005, p = 0.030, p = 0.043, respectivamente ). En condiciones de 40% -RH, los mismos parámetros fueron más altos en 80 post que en reposo (p <0,001, p = 0,001, p = 0,014, respectivamente). Al mismo tiempo, el [H2O2] EBC y el [NO2−] EBC / [NO2−] Plasma fueron más altos en 40% -RH que en 90% -RH (p = 0.010, p <0.001, respectivamente). La interacción fue significativa en FEV1 (p = 0,013) y FEF25-75% (p = 0,023), pero no en FEV1 / FVC (p = 0,362). A los 80 postes, los cambios se mantienen en 90% -RH (p <0,001), disminuyendo en 40% -RH siendo similar al reposo. Conclusión: En ciclistas recreativos, 90% -RH previene el aumento de peróxido de hidrógeno y nitrito en muestras de condensado de aire exhalado observado al 40% -RH y prolonga la broncodilatación hasta 80 post ejercicio de ciclismo.

Título traducido de la contribución	La humedad previene la formación inducida por el ejercicio de peróxido de hidrógeno y nitrito en el condensado del aire exhalado en ciclistas recreativos
Idioma original	Inglés
Páginas (desde-hasta)	2339-2348
Número de páginas	10
Publicación	European Journal of Applied Physiology
Volumen	120
N.º	10
DOI	https://doi.org/10.1007/s00421-020-04456-9
Estado	Publicada - 1 oct. 2020

Nota bibliográfica

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

Palabras clave

Broncodilatación
Ejercicio
Condensado de aliento exhalado
Humedad
Estrés oxidativo

ODS de las Naciones Unidas

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10.1007/s00421-020-04456-9

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@article{3dd635da6d96461aaeeb15bc8baaea6c,

title = "Humidity prevents the exercise-induced formation of hydrogen peroxide and nitrite in exhaled breath condensate in recreational cyclists",

abstract = "Purpose: The aerobic exercise affects the respiratory redox-state. The influence of different relative humidity (RH) levels on the formation of respiratory reactive chemical species associated with redox-state altered by exercise has been poorly explored. Our aim was to evaluate the effect of two different RH conditions (40% vs. 90%) on the concentration of hydrogen peroxide and nitrite in exhaled breath condensate ([H2O2]EBC and [NO2−]EBC) and spirometry parameters in recreational cyclists. Methods: Sixteen men and women (12/4) (mean age ± SD: 23.5 ± 2.2 years) completed 60-min of cycling at 166.3 ± 26.9 watts (70% of maximum load of V ˙ O 2-max. test, 49.3 ± 7.6 mL·min−1·kg−1) at random 40%-RH and 90%-RH conditions separated by 7 days. The two-way RM-ANOVA test was applied to compare [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma at rest and 80-min post-exercise (80-post); and spirometry parameters at rest, 20-post and 80-post. Results: The interaction of factors (humidity × time) was significant in [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma (p = 0.005, p = 0.030, p = 0.043, respectively). At 40%-RH conditions, the same parameters were higher in 80-post than at rest (p < 0.001, p = 0.001, p = 0.014, respectively). At the same time, the [H2O2]EBC and [NO2−]EBC/[NO2−]Plasma were higher in 40%-RH than 90%-RH (p = 0.010, p < 0.001, respectively). The interaction was significant in FEV1 (p = 0.013) and FEF25-75% (p = 0.023), but not in FEV1/FVC (p = 0.362). At 80-post, the changes are kept in 90%-RH (p < 0.001), diminishing in 40%-RH being similar to rest. Conclusion: In recreational cyclists, 90%-RH prevents the increase of hydrogen peroxide and nitrite in exhaled breath condensate samples observed at 40%-RH and prolonging the bronchodilation until 80-post cycling exercise.",

keywords = "Bronchodilation, Exercise, Exhaled breath condensate, Humidity, Oxidative stress, Broncodilataci{\'o}n, Ejercicio, Condensado de aliento exhalado, Humedad, Estr{\'e}s oxidativo",

author = "Felipe Contreras-Brice{\~n}o and Maximiliano Espinosa-Ramirez and Gin{\'e}s Viscor and Araneda, {Oscar F.}",

note = "Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2020",

month = oct,

day = "1",

doi = "10.1007/s00421-020-04456-9",

language = "English",

volume = "120",

pages = "2339--2348",

journal = "European Journal of Applied Physiology",

issn = "1439-6319",

publisher = "Springer Verlag",

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}

Humidity prevents the exercise-induced formation of hydrogen peroxide and nitrite in exhaled breath condensate in recreational cyclists. / Contreras-Briceño, Felipe; Espinosa-Ramirez, Maximiliano; Viscor, Ginés et al.

En: European Journal of Applied Physiology, Vol. 120, N.º 10, 01.10.2020, p. 2339-2348.

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Humidity prevents the exercise-induced formation of hydrogen peroxide and nitrite in exhaled breath condensate in recreational cyclists

AU - Contreras-Briceño, Felipe

AU - Espinosa-Ramirez, Maximiliano

AU - Viscor, Ginés

AU - Araneda, Oscar F.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Purpose: The aerobic exercise affects the respiratory redox-state. The influence of different relative humidity (RH) levels on the formation of respiratory reactive chemical species associated with redox-state altered by exercise has been poorly explored. Our aim was to evaluate the effect of two different RH conditions (40% vs. 90%) on the concentration of hydrogen peroxide and nitrite in exhaled breath condensate ([H2O2]EBC and [NO2−]EBC) and spirometry parameters in recreational cyclists. Methods: Sixteen men and women (12/4) (mean age ± SD: 23.5 ± 2.2 years) completed 60-min of cycling at 166.3 ± 26.9 watts (70% of maximum load of V ˙ O 2-max. test, 49.3 ± 7.6 mL·min−1·kg−1) at random 40%-RH and 90%-RH conditions separated by 7 days. The two-way RM-ANOVA test was applied to compare [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma at rest and 80-min post-exercise (80-post); and spirometry parameters at rest, 20-post and 80-post. Results: The interaction of factors (humidity × time) was significant in [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma (p = 0.005, p = 0.030, p = 0.043, respectively). At 40%-RH conditions, the same parameters were higher in 80-post than at rest (p < 0.001, p = 0.001, p = 0.014, respectively). At the same time, the [H2O2]EBC and [NO2−]EBC/[NO2−]Plasma were higher in 40%-RH than 90%-RH (p = 0.010, p < 0.001, respectively). The interaction was significant in FEV1 (p = 0.013) and FEF25-75% (p = 0.023), but not in FEV1/FVC (p = 0.362). At 80-post, the changes are kept in 90%-RH (p < 0.001), diminishing in 40%-RH being similar to rest. Conclusion: In recreational cyclists, 90%-RH prevents the increase of hydrogen peroxide and nitrite in exhaled breath condensate samples observed at 40%-RH and prolonging the bronchodilation until 80-post cycling exercise.

AB - Purpose: The aerobic exercise affects the respiratory redox-state. The influence of different relative humidity (RH) levels on the formation of respiratory reactive chemical species associated with redox-state altered by exercise has been poorly explored. Our aim was to evaluate the effect of two different RH conditions (40% vs. 90%) on the concentration of hydrogen peroxide and nitrite in exhaled breath condensate ([H2O2]EBC and [NO2−]EBC) and spirometry parameters in recreational cyclists. Methods: Sixteen men and women (12/4) (mean age ± SD: 23.5 ± 2.2 years) completed 60-min of cycling at 166.3 ± 26.9 watts (70% of maximum load of V ˙ O 2-max. test, 49.3 ± 7.6 mL·min−1·kg−1) at random 40%-RH and 90%-RH conditions separated by 7 days. The two-way RM-ANOVA test was applied to compare [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma at rest and 80-min post-exercise (80-post); and spirometry parameters at rest, 20-post and 80-post. Results: The interaction of factors (humidity × time) was significant in [H2O2]EBC, [NO2−]EBC, [NO2−]EBC/[NO2−]Plasma (p = 0.005, p = 0.030, p = 0.043, respectively). At 40%-RH conditions, the same parameters were higher in 80-post than at rest (p < 0.001, p = 0.001, p = 0.014, respectively). At the same time, the [H2O2]EBC and [NO2−]EBC/[NO2−]Plasma were higher in 40%-RH than 90%-RH (p = 0.010, p < 0.001, respectively). The interaction was significant in FEV1 (p = 0.013) and FEF25-75% (p = 0.023), but not in FEV1/FVC (p = 0.362). At 80-post, the changes are kept in 90%-RH (p < 0.001), diminishing in 40%-RH being similar to rest. Conclusion: In recreational cyclists, 90%-RH prevents the increase of hydrogen peroxide and nitrite in exhaled breath condensate samples observed at 40%-RH and prolonging the bronchodilation until 80-post cycling exercise.

KW - Bronchodilation

KW - Exercise

KW - Exhaled breath condensate

KW - Humidity

KW - Oxidative stress

KW - Broncodilatación

KW - Ejercicio

KW - Condensado de aliento exhalado

KW - Humedad

KW - Estrés oxidativo

UR - http://www.scopus.com/inward/record.url?scp=85089158907&partnerID=8YFLogxK

U2 - 10.1007/s00421-020-04456-9

DO - 10.1007/s00421-020-04456-9

M3 - Article

C2 - 32772245

AN - SCOPUS:85089158907

SN - 1439-6319

VL - 120

SP - 2339

EP - 2348

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

IS - 10

ER -

Humidity prevents the exercise-induced formation of hydrogen peroxide and nitrite in exhaled breath condensate in recreational cyclists

Resumen

Nota bibliográfica

Palabras clave

ODS de las Naciones Unidas

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