Human decidua: a source of cachectin-tumor necrosis factor

Roberto Romero, Moshe Mazor, Kirk Manogue, Enrique Oyarzun, Anthony Cerami

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

68 Citas (Scopus)

Resumen

Cachectin-tumor necrosis factor (TNF-α) has been implicated as a possible signal for the initiation of human parturition in the setting of infection. These studies were conducted to determine whether human decidua can produce TNF-α in response to bacterial lipopolysaccharide (LPS). Decidual explants from women undergoing elective cesarean sections were incubated with and without Escherichia coli LPS (25 ng/ml) for 20 h. TNF-α concentration in the conditioned media was measured with an enzyme-linked immunoassay and bioassay (L929 bioassay). While conditioned media from unstimulated decidual explants contained either undetectable or low levels of TNF-α, conditioned media from LPS stimulated decidua contained TNF-α (mean = 2.6 pmol/mg protein per 20 hours, SEM ± 1.03). There was a strong correlation between the immunoreactive and bioactive TNF-α (Spearman rank correlation r = 0.76, P < 0.001). We conclude that human decidua in vitro can produce TNF-α in response to LPS.

Idioma originalInglés
Páginas (desde-hasta)123-127
Número de páginas5
PublicaciónEuropean Journal of Obstetrics, Gynecology and Reproductive Biology
Volumen41
N.º2
DOI
EstadoPublicada - 13 sep 1991
Publicado de forma externa

Huella

Profundice en los temas de investigación de 'Human decidua: a source of cachectin-tumor necrosis factor'. En conjunto forman una huella única.

Citar esto