TY - JOUR
T1 - Exosome-like vesicles in Apis mellifera bee pollen, honey and royal jelly contribute to their antibacterial and pro-regenerative activity
AU - Schuh, Christina M.A.P.
AU - Aguayo, Sebastian
AU - Zavala, Gabriela
AU - Khoury, Maroun
N1 - Funding Information:
This work was supported by CONICYT FONDECYT (Comisión Nacional de Investigación Cientıf́ ica y Tecnológica – Fondo Nacional de Desarrollo Cientıf́ ico y Tecnológico) grant no. 11180406 and 11180101.
Publisher Copyright:
© 2019 Published by The Company of Biologists Ltd | Journal of Experimental Biology.
PY - 2019
Y1 - 2019
N2 - Microvesicles are key players in cellular communication. As glandular secretions present a rich source of active exosomes, we hypothesized that exosome-like vesicles are present in Apis mellifera hypopharyngeal gland secretomal products (honey, royal jelly and bee pollen), and participate in their known antibacterial and pro-regenerative effects. We developed an isolation protocol based on serial centrifugation and ultracentrifugation steps and demonstrated the presence of protein-containing exosome-like vesicles in all three bee-derived products. Assessing their antibacterial properties, we found that exosome-like vesicles had bacteriostatic, bactericidal and biofilm-inhibiting effects on Staphylococcus aureus. Furthermore, we demonstrated that mesenchymal stem cells (MSCs) internalize bee-derived exosomelike vesicles and that these vesicles influence the migration potential of the MSCs. In an in vitro wound-healing assay, honey and royal jelly exosome-like vesicles increased migration of human MSCs, demonstrating their inter-kingdom activity. In summary, we have discovered exosome-like vesicles as a new, active compound in bee pollen, honey and royal jelly.
AB - Microvesicles are key players in cellular communication. As glandular secretions present a rich source of active exosomes, we hypothesized that exosome-like vesicles are present in Apis mellifera hypopharyngeal gland secretomal products (honey, royal jelly and bee pollen), and participate in their known antibacterial and pro-regenerative effects. We developed an isolation protocol based on serial centrifugation and ultracentrifugation steps and demonstrated the presence of protein-containing exosome-like vesicles in all three bee-derived products. Assessing their antibacterial properties, we found that exosome-like vesicles had bacteriostatic, bactericidal and biofilm-inhibiting effects on Staphylococcus aureus. Furthermore, we demonstrated that mesenchymal stem cells (MSCs) internalize bee-derived exosomelike vesicles and that these vesicles influence the migration potential of the MSCs. In an in vitro wound-healing assay, honey and royal jelly exosome-like vesicles increased migration of human MSCs, demonstrating their inter-kingdom activity. In summary, we have discovered exosome-like vesicles as a new, active compound in bee pollen, honey and royal jelly.
KW - Apis mellifera
KW - Bactericide
KW - Inter-kingdom communication
KW - Intercellular communication
KW - Microvesicle
KW - Apis mellifera
KW - Bactericide
KW - Inter-kingdom communication
KW - Intercellular communication
KW - Microvesicle
UR - http://www.scopus.com/inward/record.url?scp=85073490155&partnerID=8YFLogxK
U2 - 10.1242/jeb.208702
DO - 10.1242/jeb.208702
M3 - Article
C2 - 31537653
AN - SCOPUS:85073490155
SN - 0022-0949
VL - 222
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
IS - 20
M1 - jeb.208702
ER -