Evaluation of mesenchymal stem cell proliferation using different low intensity pulsed ultrasound intensities

Daniela Becerra, Z. Jaime Díaz, A. Flavio Carrión, Carolina Inostroza, W. Rodrigo Oyonarte*

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.
Título traducido de la contribuciónEvaluation of mesenchymal stem cell proliferation using different low intensity pulsed ultrasound intensities
Idioma originalEspañol
Páginas (desde-hasta)621-626
Número de páginas6
PublicaciónInternational Journal of Morphology
Volumen30
N.º2
DOI
EstadoPublicada - jun 2012

Palabras clave

  • Adult stem cells
  • Mesenchymal stem cells
  • Ultrasound

Huella

Profundice en los temas de investigación de 'Evaluation of mesenchymal stem cell proliferation using different low intensity pulsed ultrasound intensities'. En conjunto forman una huella única.

Citar esto