Evaluación de la Susceptibilidad Antimicrobiana de Porphyromonas gingivalis Aisladas de Pacientes Periodontales en Población Chilena

Lorena Isbej; Natacha Oyarzo; María José Contreras; Duniel Ortuño Borroto; Marusella Lam; Patricia García

doi:10.4067/S0718-381X2022000200279

Evaluación de la Susceptibilidad Antimicrobiana de Porphyromonas gingivalis Aisladas de Pacientes Periodontales en Población Chilena

Lorena Isbej^*, Natacha Oyarzo, María José Contreras, Duniel Ortuño Borroto, Marusella Lam, Patricia García

^*Autor correspondiente de este trabajo

Facultad de Odontología

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Resumen

Porphyromonas gingivalis (P. gingivalis) es un microorganismo frecuentemente aislado en pacientes con periodontitis, en los que también se incluye los de la población chilena. El modelo de "Keystone bacteria" demostró que P. gingivalis induce la disbiosis del biofilm subgingival y permite el desarrollo de algunas especies sobre otras, modulando la patogenicidad de la comunidad microbiológica completa. El tratamiento de la periodontitis es principalmente mecánico, pero en condiciones específicas es necesario el complemento con antibioterapia. Los estudios globales de antibióticos evaluados en ensayos clínicos y estudios in vitro han mostrado resultados mixtos en cuanto a eficacia y susceptibilidad. Este estudio descriptivo tuvo como objetivo evaluar el perfil de susceptibilidad antimicrobiana in vitro a metronidazol, clindamicina, amoxicilina más ácido clavulánico, moxifloxacino y azitromicina de P. gingivalis aisladas de pacientes periodontales chilenos. Se obtuvieron muestras microbiológicas de pacientes con diagnóstico de periodontitis estadíos III y IV generalizada, las que se sometieron a procesos de identificación mediante un espectrómetro de masas (MALDI-TOF MS). Posteriormente, a cada muestra positiva a P. gingivalis se aplicó el protocolo gold standard de susceptibilidad para los cinco antimicrobianos evaluados (Dilución en Agar Sangre Brucella- McFarland 0.5). Se seleccionaron 50 pacientes (25 mujeres, 25 hombres) entre 34-69 años. Finalmente, se recuperaron 25 cepas de P. gingivalis (50 %) para el análisis de susceptibilidad y todas ellas fueron sensibles a todos los antibióticos evaluados (100 % susceptibilidad). Las cepas de P. gingivalis fueron altamente sensibles a los cinco antibióticos evaluados en esta población, lo que podría implicar contar con diferentes alternativas de tratamiento farmacológico antimicrobi ano como complemento al tratamiento mecánico convencional en pacientes específicos.

Título traducido de la contribución	Evaluation of the Antimicrobial Susceptibility of Porphyromonas gingivalis Isolated From Periodontal Patients in Chilean Population
Idioma original	Español
Páginas (desde-hasta)	279-284
Número de páginas	6
Publicación	International journal of odontostomatology
Volumen	16
N.º	2
DOI	https://doi.org/10.4067/S0718-381X2022000200279
Estado	Publicada - jun. 2022

Palabras clave

Porphyromonas gingivalis
periodontitis
resistencia antimicrobiana
pruebas de susceptibilidad antimicrobiana

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10.4067/S0718-381X2022000200279Licencia: CC BY-NC

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@article{75395ed09aab48be82ff6238a613b9c3,

title = "Evaluaci{\'o}n de la Susceptibilidad Antimicrobiana de Porphyromonas gingivalis Aisladas de Pacientes Periodontales en Poblaci{\'o}n Chilena",

abstract = "Porphyromonas gingivalis (P. gingivalis) is a microorganism frequently isolated in patients with periodontitis, which also include those of Chilean population. The {"}Keystone bacteria{"} model demonstrated that P. gingivalis induces dysbiosis of the subgingival biofilm and allows the development of some species over others, modulating the pathogenicity of the entire microbiological community. The treatment of periodontitis is mainly mechanical; nevertheless, under specific conditions the complement with antibiotherapy is needed. Global studies of antibiotics evaluated in clinical trials and in vitro studies have shown mixed results in terms of efficacy and susceptibility. This descriptive study aimed to evaluate antimicrobial susceptibility profile in vitro to metronidazole, clindamycin, amoxicillin plus clavulanic acid, moxifloxacin and azithromycin of P. gingivalis isolated from Chilean periodontal patients. Microbiological samples were obtained from patients with a diagnosis of generalized periodontitis stages III and IV, which were exposed to identification processes by a mass spectrometer (MALDI-TOF MS). Subsequently, the gold standard susceptibility protocol for the five antimicrobials evaluated was applied to each P. gingivalis- positive sample (Dilution in Brucella-McFarland Blood Agar 0.5). 50 patients (25 women, 25 men) between 34-69 years old were selected. Finally, 25 P. gingivalis strains (50 %) were recovered for susceptibility testing and all of them were susceptible to all antibiotics tested (100 % susceptibility). P. gingivalis strains were highly susceptible to the five antibiotics evaluated in this population, which could implies counting different antimicrobial pharmacological treatment alternatives as a complement to conventional mechanical treatment in specific patients.",

keywords = "Porphyromonas gingivalis, periodontitis, drug resistance, bacterial, microbial sensitivity tests, Porphyromonas gingivalis, periodontitis, resistencia antimicrobiana, pruebas de susceptibilidad antimicrobiana",

author = "Lorena Isbej and Natacha Oyarzo and Contreras, {Mar{\'i}a Jos{\'e}} and {Ortu{\~n}o Borroto}, Duniel and Marusella Lam and Patricia Garc{\'i}a",

year = "2022",

month = jun,

doi = "10.4067/S0718-381X2022000200279",

language = "Spanish",

volume = "16",

pages = "279--284",

journal = "International journal of odontostomatology",

issn = "0718-381X",

publisher = "Universidad de la Frontera",

number = "2",

}

Evaluación de la Susceptibilidad Antimicrobiana de Porphyromonas gingivalis Aisladas de Pacientes Periodontales en Población Chilena. / Isbej, Lorena; Oyarzo, Natacha; Contreras, María José et al.
En: International journal of odontostomatology, Vol. 16, N.º 2, 06.2022, p. 279-284.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Evaluación de la Susceptibilidad Antimicrobiana de Porphyromonas gingivalis Aisladas de Pacientes Periodontales en Población Chilena

AU - Isbej, Lorena

AU - Oyarzo, Natacha

AU - Contreras, María José

AU - Ortuño Borroto, Duniel

AU - Lam, Marusella

AU - García, Patricia

PY - 2022/6

Y1 - 2022/6

N2 - Porphyromonas gingivalis (P. gingivalis) is a microorganism frequently isolated in patients with periodontitis, which also include those of Chilean population. The "Keystone bacteria" model demonstrated that P. gingivalis induces dysbiosis of the subgingival biofilm and allows the development of some species over others, modulating the pathogenicity of the entire microbiological community. The treatment of periodontitis is mainly mechanical; nevertheless, under specific conditions the complement with antibiotherapy is needed. Global studies of antibiotics evaluated in clinical trials and in vitro studies have shown mixed results in terms of efficacy and susceptibility. This descriptive study aimed to evaluate antimicrobial susceptibility profile in vitro to metronidazole, clindamycin, amoxicillin plus clavulanic acid, moxifloxacin and azithromycin of P. gingivalis isolated from Chilean periodontal patients. Microbiological samples were obtained from patients with a diagnosis of generalized periodontitis stages III and IV, which were exposed to identification processes by a mass spectrometer (MALDI-TOF MS). Subsequently, the gold standard susceptibility protocol for the five antimicrobials evaluated was applied to each P. gingivalis- positive sample (Dilution in Brucella-McFarland Blood Agar 0.5). 50 patients (25 women, 25 men) between 34-69 years old were selected. Finally, 25 P. gingivalis strains (50 %) were recovered for susceptibility testing and all of them were susceptible to all antibiotics tested (100 % susceptibility). P. gingivalis strains were highly susceptible to the five antibiotics evaluated in this population, which could implies counting different antimicrobial pharmacological treatment alternatives as a complement to conventional mechanical treatment in specific patients.

AB - Porphyromonas gingivalis (P. gingivalis) is a microorganism frequently isolated in patients with periodontitis, which also include those of Chilean population. The "Keystone bacteria" model demonstrated that P. gingivalis induces dysbiosis of the subgingival biofilm and allows the development of some species over others, modulating the pathogenicity of the entire microbiological community. The treatment of periodontitis is mainly mechanical; nevertheless, under specific conditions the complement with antibiotherapy is needed. Global studies of antibiotics evaluated in clinical trials and in vitro studies have shown mixed results in terms of efficacy and susceptibility. This descriptive study aimed to evaluate antimicrobial susceptibility profile in vitro to metronidazole, clindamycin, amoxicillin plus clavulanic acid, moxifloxacin and azithromycin of P. gingivalis isolated from Chilean periodontal patients. Microbiological samples were obtained from patients with a diagnosis of generalized periodontitis stages III and IV, which were exposed to identification processes by a mass spectrometer (MALDI-TOF MS). Subsequently, the gold standard susceptibility protocol for the five antimicrobials evaluated was applied to each P. gingivalis- positive sample (Dilution in Brucella-McFarland Blood Agar 0.5). 50 patients (25 women, 25 men) between 34-69 years old were selected. Finally, 25 P. gingivalis strains (50 %) were recovered for susceptibility testing and all of them were susceptible to all antibiotics tested (100 % susceptibility). P. gingivalis strains were highly susceptible to the five antibiotics evaluated in this population, which could implies counting different antimicrobial pharmacological treatment alternatives as a complement to conventional mechanical treatment in specific patients.

KW - Porphyromonas gingivalis

KW - periodontitis

KW - drug resistance

KW - bacterial

KW - microbial sensitivity tests

KW - Porphyromonas gingivalis

KW - periodontitis

KW - resistencia antimicrobiana

KW - pruebas de susceptibilidad antimicrobiana

U2 - 10.4067/S0718-381X2022000200279

DO - 10.4067/S0718-381X2022000200279

M3 - Article

SN - 0718-381X

VL - 16

SP - 279

EP - 284

JO - International journal of odontostomatology

JF - International journal of odontostomatology

IS - 2

ER -

Evaluación de la Susceptibilidad Antimicrobiana de Porphyromonas gingivalis Aisladas de Pacientes Periodontales en Población Chilena

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