Resumen
Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.
Título traducido de la contribución | Evaluation of mesenchymal stem cell proliferation using different low intensity pulsed ultrasound intensities |
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Idioma original | Español |
Páginas (desde-hasta) | 621-626 |
Número de páginas | 6 |
Publicación | International Journal of Morphology |
Volumen | 30 |
N.º | 2 |
DOI | |
Estado | Publicada - jun. 2012 |
Palabras clave
- Adult stem cells
- Mesenchymal stem cells
- Ultrasound