Effect of different protocols in preconditioning with EDTA in sclerotic dentin and enamel before universal adhesives applied in self-etch mode

Objectives: The aim of this study was to investigate the effect of different protocols of 17% ethylene diamine tetra-acetic acid (EDTA) conditioning on the etching pattern and immediate bond strength of universal adhesives to enamel and sclerotic dentin. Methods and Materials: Forty bovine teeth with sclerotic dentin and 20 human third molars were randomly divided into eight groups resulting from the combination of the main factors surface treatment (none, twominute EDTA conditioning manual application, 30-second EDTA manual application, 30-second EDTA sonic application) and adhesives systems (Scotchbond Universal Adhesive [SBU] and Prime &Bond Elect [PBE]). Resindentin and enamel-dentin bond specimens were prepared and tested under the micro.