TY - JOUR
T1 - Early pregnancy levels of gingival crevicular fluid matrix metalloproteinases-8 and -9 are associated with the severity of periodontitis and the development of gestational diabetes mellitus
AU - Chaparro, Alejandra
AU - Realini, Ornella
AU - Hernández, Marcela
AU - Albers, Daniela
AU - Weber, Laura
AU - Ramírez, Valeria
AU - Param, Fernanda
AU - Kusanovic, Juan Pedro
AU - Sorsa, Timo
AU - Rice, Gregory Edward
AU - Illanes, Sebastián E.
N1 - Funding Information:
FONDEF IDeA ID16I10452, Fund to Encourage Scientific and Technological Development (“FONDEF”), located at Moneda 1375, Santiago, Chile. Timo Sorsa has received grants from the Helsinki University Hospital (TYH2016251, TYH2017251, TYH2018229, TYH2019319, Y1014SLO17, Y1014SLO18 and, Y1014SULE1) and Karolinska Institutet, Stockholm, Sweden.
Publisher Copyright:
© 2020 American Academy of Periodontology
PY - 2021/2/1
Y1 - 2021/2/1
N2 - Background: Gestational diabetes mellitus (GDM) is increasing worldwide and women with a history of GDM are at risk of developing type 2 diabetes which is a risk factor for periodontitis. The aim of this study was to explore the association between the concentrations of matrix metalloproteinase (MMP)-8 and -9 in gingival crevicular fluid (GCF) during early pregnancy with the periodontal diagnosis and the risk of GDM development. Methods: A prospective cohort study, including 314 women, enrolled at 11 to 14 weeks of pregnancy was conducted. A complete maternal/obstetric and periodontal exam was performed, and GCF samples were obtained for the MMP-8 and -9 determination by Multiplex Elisa Assays. Mann-Whitney test; Spearman's correlation and log-binomial regression model estimated the association between MMPs concentration in GCF and GDM. Results: Fourteen percent of the pregnancies were diagnosed with GDM. An increase in the concentration of MMP-8 and -9 in women with periodontitis stage III and IV compared to periodontitis stage I was observed (99.31 ng/mL [IQR: 85.32] versus 71.95 ng/mL [IQR: 54.04], and 262.4 ng/mL [IQR: 312.55] versus 114.1 ng/mL [IQR: 184.94], respectively). Women who developed GDM showed increased concentrations of MMP-8 and -9 in GCF since the beginning of pregnancy (P = 0.0381; P = 0.0302, respectively). MMP-8 concentration in GCF was associated with GDM (RR: 1.19; P = 0.045; CI 95% 1.00 to 1.40; and RR: 1.20; P = 0.063; CI 95% 0.99 to 1.45 in the adjusted model). Conclusion(s): GCF concentrations of MMP-8 and -9 at early of pregnancy are increased in women with severe periodontitis and associated with the GDM development.
AB - Background: Gestational diabetes mellitus (GDM) is increasing worldwide and women with a history of GDM are at risk of developing type 2 diabetes which is a risk factor for periodontitis. The aim of this study was to explore the association between the concentrations of matrix metalloproteinase (MMP)-8 and -9 in gingival crevicular fluid (GCF) during early pregnancy with the periodontal diagnosis and the risk of GDM development. Methods: A prospective cohort study, including 314 women, enrolled at 11 to 14 weeks of pregnancy was conducted. A complete maternal/obstetric and periodontal exam was performed, and GCF samples were obtained for the MMP-8 and -9 determination by Multiplex Elisa Assays. Mann-Whitney test; Spearman's correlation and log-binomial regression model estimated the association between MMPs concentration in GCF and GDM. Results: Fourteen percent of the pregnancies were diagnosed with GDM. An increase in the concentration of MMP-8 and -9 in women with periodontitis stage III and IV compared to periodontitis stage I was observed (99.31 ng/mL [IQR: 85.32] versus 71.95 ng/mL [IQR: 54.04], and 262.4 ng/mL [IQR: 312.55] versus 114.1 ng/mL [IQR: 184.94], respectively). Women who developed GDM showed increased concentrations of MMP-8 and -9 in GCF since the beginning of pregnancy (P = 0.0381; P = 0.0302, respectively). MMP-8 concentration in GCF was associated with GDM (RR: 1.19; P = 0.045; CI 95% 1.00 to 1.40; and RR: 1.20; P = 0.063; CI 95% 0.99 to 1.45 in the adjusted model). Conclusion(s): GCF concentrations of MMP-8 and -9 at early of pregnancy are increased in women with severe periodontitis and associated with the GDM development.
KW - Diabetes Mellitus, Type 2
KW - Diabetes, Gestational
KW - Female
KW - Gingival Crevicular Fluid
KW - Humans
KW - Matrix Metalloproteinase 8
KW - Periodontitis/complications
KW - Pregnancy
KW - Prospective Studies
UR - http://www.scopus.com/inward/record.url?scp=85091218395&partnerID=8YFLogxK
U2 - 10.1002/JPER.19-0743
DO - 10.1002/JPER.19-0743
M3 - Article
C2 - 32789908
AN - SCOPUS:85091218395
SN - 0022-3492
VL - 92
SP - 205
EP - 215
JO - Journal of Periodontology
JF - Journal of Periodontology
IS - 2
ER -