DNA extraction from formalin-fixed laryngeal biopsies: Comparison of techniques

Mariela C. Torrente, Carolina Ríos, Carlos Misad, Roxana Ramírez, Monica Acuña, Lucia Cifuentes

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

13 Citas (Scopus)


Conclusion: PCR-quality DNA could be extracted from formalin-fixed paraffin-embedded (FFPE) samples with amplicons of at least 390 bp. Paraffin removal was not a necessary step. Proteinase K digestion was as efficient as the commercial kit for DNA extraction with a lower cost. Objectives: To compare different DNA extraction protocols for FFPE samples and to describe the suitability of the extracted DNA for PCR reactions. Methods: For deparaffinization the following techniques were compared: alkaline heat, xylene, and no removal. For DNA extraction, proteinase K digestion and organic extraction were compared. A commercial extraction kit was included as standard. DNA quality was assessed by PCR amplification of the HFE gene, for amplicons of 208 and 390 bp. Results: Extraction with the commercial kit and proteinase K digestion were more efficient than other techniques, with no statistical difference between them for both amplicons. The proteinase K digestion buffer had a cost of U$ 0.2 per sample and the commercial kit of U$7 per sample.

Idioma originalInglés
Páginas (desde-hasta)330-333
Número de páginas4
PublicaciónActa Oto-Laryngologica
EstadoPublicada - mar. 2011
Publicado de forma externa


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