Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Huabao Zheng; Daniel Kahakeaw; Juan Pablo Acevedo; Manfred T. Reetz

doi:10.1002/cctc.201000122

Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

Huabao Zheng
, Daniel Kahakeaw
, Juan Pablo Acevedo
, Manfred T. Reetz^*

^*Autor correspondiente de este trabajo

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

19 Citas (Scopus)

Resumen

Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.

Idioma original	Inglés
Páginas (desde-hasta)	958-961
Número de páginas	4
Publicación	ChemCatChem
Volumen	2
N.º	8
DOI	https://doi.org/10.1002/cctc.201000122
Estado	Publicada - 9 ago. 2010

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title = "Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide",

abstract = "Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 \% conversion and 99 \% enantiomeric excess.",

keywords = "Alcohols, Directed evolution, Enantioselectivity, Enzymes, Hydrolases",

author = "Huabao Zheng and Daniel Kahakeaw and Acevedo, \{Juan Pablo\} and Reetz, \{Manfred T.\}",

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AU - Kahakeaw, Daniel

AU - Acevedo, Juan Pablo

AU - Reetz, Manfred T.

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AB - Highly evolved: The Aspergillus niger epoxide hydrolase (ANEH) has been subjected to laboratory evolution with the creation of mutants that transform the racemic epoxide to a single stereoisomer with greater than 90 % conversion and 99 % enantiomeric excess.

KW - Alcohols

KW - Directed evolution

KW - Enantioselectivity

KW - Enzymes

KW - Hydrolases

UR - https://www.scopus.com/pages/publications/78149264575

U2 - 10.1002/cctc.201000122

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Directed Evolution of Enantioconvergency: the Case of an Epoxide Hydrolase-Catalyzed Reaction of a Racemic Epoxide

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