TY - JOUR
T1 - Designing a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineering
AU - Enrione, Javier
AU - Díaz-Calderón, Paulo
AU - Weinstein-Oppenheimer, Caroline R.
AU - Sánchez, Elizabeth
AU - Fuentes, Miguel A.
AU - Brown, Donald I.
AU - Herrera, Hugo
AU - Acevedo, Cristian A.
PY - 2013/11
Y1 - 2013/11
N2 - Cell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.
AB - Cell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.
KW - Biopolymeric scaffold
KW - Chitosan
KW - Gelatin
KW - Hyaluronic acid
KW - Thermophysical properties
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=84892435644&partnerID=8YFLogxK
U2 - 10.1007/s00449-013-0971-x
DO - 10.1007/s00449-013-0971-x
M3 - Article
C2 - 23708649
AN - SCOPUS:84892435644
SN - 1615-7591
VL - 36
SP - 1947
EP - 1956
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 12
ER -