Deficient Vitamin E Uptake during Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI

SR-BI is the main receptor for high density lipoproteins (HDL) and mediates the bidirectional transport of lipids, such as cholesterol and Vitamin E, between these particles and cells. During early development, SR-BI is expressed in extraembryonic tissue, specifically in trophoblast giant cells in the parietal yolk sac. We previously showed that approximately 50% of SR-BI^-/- embryos fail to close the anterior neural tube and develop exencephaly, a perinatal lethal condition. Here, we evaluated the role of SR-BI in embryonic Vitamin E uptake during murine neural tube closure. Our results showed that SR-BI^-/- embryos had a very low Vitamin E content in comparison to SR-BI^+/+ embryos. Whereas SR-BI^-/- embryos with closed neural tubes (nSR-BI^-/-) had high levels of reactive oxygen species (ROS), intermediate ROS levels between SR-BI^+/+ and nSR-BI^-/- embryos were detected in SR-BI^-/- with NTD (NTD SR-BI^-/-). Reduced expression of Pax3, Alx1 and Alx3 genes was found in NTD SR-BI^-/- embryos. Maternal α-tocopherol dietary supplementation prevented NTD almost completely (from 54% to 2%, p < 0.001) in SR-BI^-/- embryos and normalized ROS and gene expression levels. In sum, our results suggest the involvement of SR-BI in the maternal provision of embryonic Vitamin E to the mouse embryo during neural tube closure.