Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease

Keyphrases

• Antarctic Bacteria 33%
• Autocatalytic Cleavage 33%
• Bacterial Genera 33%
• Biotechnological Applications 33%
• Cloning Expression 100%
• Cold Adaptation 100%
• Cold Environment 33%
• Cold Substrate 33%
• Cold-adapted Protease 100%
• Evolutionary Success 33%
• Genome Walking 33%
• Heat-induced 33%
• High Catalytic Activity 33%
• Homology Modeling 33%
• Low Melting Point 33%
• Low Temperature 33%
• Maximum Activity 33%
• Mesophilic 33%
• Optimum pH 33%
• Potentiation 33%
• Precursor Protein 33%
• Protease 100%
• Site-directed mutagenesis 33%
• Substrate Specificity 33%
• Subtilisin-like 100%
• Thermolabile 100%
• Walking Methods 33%

Biochemistry, Genetics and Molecular Biology

• Catalytic Efficiency 20%
• Cold Acclimatization 100%
• Enzyme 40%
• Enzyme Specificity 20%
• Evolution 20%
• Genetics 20%
• Melting Point 20%
• Primer Walking 20%
• Protease 100%
• Protein Precursor 20%
• Site-Directed Mutagenesis 20%
• Subtilisin 100%