Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature

Ilaria Del Gaudio; Ivana Sreckovic; Pablo Zardoya-Laguardia; Eva Bernhart; Christina Christoffersen; Saša Frank; Gunther Marsche; Sebastian E. Illanes; Christian Wadsack

doi:10.1016/j.bbalip.2020.158632

Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature

Título traducido de la contribución: El complejo HDL-S1P de sangre de cordón circulante preserva la integridad de la vasculatura fetoplacentaria

Ilaria Del Gaudio, Ivana Sreckovic, Pablo Zardoya-Laguardia, Eva Bernhart, Christina Christoffersen, Saša Frank, Gunther Marsche, Sebastian E. Illanes, Christian Wadsack^*

^*Autor correspondiente de este trabajo

Claustro Doctorado en Biomedicina

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

10 Citas (Scopus)

Resumen

Las morbilidades de la descendencia perinatal y a largo plazo dependen en gran medida de la preservación de la homeostasis vascular placentaria durante el embarazo. En adultos, el complejo HDL-apoM-S1P protege el endotelio y mantiene la integridad vascular. Sin embargo, el metabolismo y la biología de las HDL derivadas de la sangre del cordón umbilical (denominadas HDL neonatal, nHDL) difieren notablemente de las de los adultos. Aquí, investigamos el papel de las HDL neonatales en la regulación de la función vascular placentaria. Demostramos que nHDL es un portador principal de esfingosina-1-fosfato (S1P), que está anclado a la partícula a través de apoM (rs = 0,90, p <0,0001) en la circulación fetal. Además, este complejo interactúa con los receptores S1P en el endotelio fetoplacentario y activa las proteínas quinasas 1 y 2 reguladas por señales extracelulares (ERK) y la fosfolipasa C (PLC) aguas abajo, promueve la proliferación de células endoteliales y el flujo de calcio. En particular, el complejo nHDL-S1P desencadena la reorganización de los filamentos de actina, lo que conduce a una mejora de la función de barrera endotelial placentaria. Además, nHDL induce vasorrelajación de arterias coriónicas placentarias aisladas. Tomados en conjunto, estos resultados sugieren que la nHDL circulante ejerce efectos vasoprotectores sobre la barrera endotelial fetoplacentaria principalmente a través de la señalización S1P.

Título traducido de la contribución	El complejo HDL-S1P de sangre de cordón circulante preserva la integridad de la vasculatura fetoplacentaria
Idioma original	Inglés
Número de artículo	158632
Publicación	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volumen	1865
N.º	4
DOI	https://doi.org/10.1016/j.bbalip.2020.158632
Estado	Publicada - abr. 2020

Nota bibliográfica

Publisher Copyright:
© 2020 The Author(s)

Palabras clave

Lípidos bioactivos
Lipoproteína de alta densidad
Placenta humana
Endotelio vascular placentario
El embarazo

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10.1016/j.bbalip.2020.158632

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Del Gaudio, I., Sreckovic, I., Zardoya-Laguardia, P., Bernhart, E., Christoffersen, C., Frank, S., Marsche, G., Illanes, S. E., & Wadsack, C. (2020). Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1865(4), [158632]. https://doi.org/10.1016/j.bbalip.2020.158632

Del Gaudio, Ilaria ; Sreckovic, Ivana ; Zardoya-Laguardia, Pablo ; Bernhart, Eva ; Christoffersen, Christina ; Frank, Saša ; Marsche, Gunther ; Illanes, Sebastian E. ; Wadsack, Christian. / Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature. En: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2020 ; Vol. 1865, N.º 4.

@article{39953b07663048c8a53ae370bcce4513,

title = "Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature",

abstract = "Perinatal and long-term offspring morbidities are strongly dependent on the preservation of placental vascular homeostasis during pregnancy. In adults, the HDL-apoM-S1P complex protects the endothelium and maintains vascular integrity. However, the metabolism and biology of cord blood-derived HDLs (referred to as neonatal HDL, nHDL) strikingly differ from those in adults. Here, we investigate the role of neonatal HDLs in the regulation of placental vascular function. We show that nHDL is a major carrier of sphingosine-1-phosphate (S1P), which is anchored to the particle through apoM (rs = 0.90, p < 0.0001) in the fetal circulation. Furthermore, this complex interacts with S1P receptors on the feto-placental endothelium and activates specifically extracellular signal-regulated protein kinases 1 and 2 (ERK) and phospholipase C (PLC) downstream signaling, promotes endothelial cell proliferation and calcium flux. Notably, the nHDL-S1P complex triggers actin filaments reorganization, leading to an enhancement of placental endothelial barrier function. Additionally, nHDL induces vasorelaxation of isolated placental chorionic arteries. Taken together, these results suggest that circulating nHDL exerts vasoprotective effects on the feto-placental endothelial barrier mainly via S1P signaling.",

keywords = "Bioactive lipids, High-density lipoprotein, Human placenta, Placental vascular endothelium, Pregnancy, L{\'i}pidos bioactivos, Lipoprote{\'i}na de alta densidad, Placenta humana, Endotelio vascular placentario, El embarazo",

author = "{Del Gaudio}, Ilaria and Ivana Sreckovic and Pablo Zardoya-Laguardia and Eva Bernhart and Christina Christoffersen and Sa{\v s}a Frank and Gunther Marsche and Illanes, {Sebastian E.} and Christian Wadsack",

note = "Funding Information: The authors thank Susanne Kopp for excellent technical support. I. Del Gaudio, I. Sreckovic and P. Zardoya-Laguardia received funding from the Medical University of Graz within the PhD Program Molecular Medicine. Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = apr,

doi = "10.1016/j.bbalip.2020.158632",

language = "English",

volume = "1865",

journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",

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Del Gaudio, I, Sreckovic, I, Zardoya-Laguardia, P, Bernhart, E, Christoffersen, C, Frank, S, Marsche, G, Illanes, SE & Wadsack, C 2020, 'Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, vol. 1865, n.º 4, 158632. https://doi.org/10.1016/j.bbalip.2020.158632

Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature. / Del Gaudio, Ilaria; Sreckovic, Ivana; Zardoya-Laguardia, Pablo; Bernhart, Eva; Christoffersen, Christina; Frank, Saša; Marsche, Gunther; Illanes, Sebastian E.; Wadsack, Christian.

En: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1865, N.º 4, 158632, 04.2020.

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature

AU - Del Gaudio, Ilaria

AU - Sreckovic, Ivana

AU - Zardoya-Laguardia, Pablo

AU - Bernhart, Eva

AU - Christoffersen, Christina

AU - Frank, Saša

AU - Marsche, Gunther

AU - Illanes, Sebastian E.

AU - Wadsack, Christian

N1 - Funding Information: The authors thank Susanne Kopp for excellent technical support. I. Del Gaudio, I. Sreckovic and P. Zardoya-Laguardia received funding from the Medical University of Graz within the PhD Program Molecular Medicine. Publisher Copyright: © 2020 The Author(s)

PY - 2020/4

Y1 - 2020/4

N2 - Perinatal and long-term offspring morbidities are strongly dependent on the preservation of placental vascular homeostasis during pregnancy. In adults, the HDL-apoM-S1P complex protects the endothelium and maintains vascular integrity. However, the metabolism and biology of cord blood-derived HDLs (referred to as neonatal HDL, nHDL) strikingly differ from those in adults. Here, we investigate the role of neonatal HDLs in the regulation of placental vascular function. We show that nHDL is a major carrier of sphingosine-1-phosphate (S1P), which is anchored to the particle through apoM (rs = 0.90, p < 0.0001) in the fetal circulation. Furthermore, this complex interacts with S1P receptors on the feto-placental endothelium and activates specifically extracellular signal-regulated protein kinases 1 and 2 (ERK) and phospholipase C (PLC) downstream signaling, promotes endothelial cell proliferation and calcium flux. Notably, the nHDL-S1P complex triggers actin filaments reorganization, leading to an enhancement of placental endothelial barrier function. Additionally, nHDL induces vasorelaxation of isolated placental chorionic arteries. Taken together, these results suggest that circulating nHDL exerts vasoprotective effects on the feto-placental endothelial barrier mainly via S1P signaling.

AB - Perinatal and long-term offspring morbidities are strongly dependent on the preservation of placental vascular homeostasis during pregnancy. In adults, the HDL-apoM-S1P complex protects the endothelium and maintains vascular integrity. However, the metabolism and biology of cord blood-derived HDLs (referred to as neonatal HDL, nHDL) strikingly differ from those in adults. Here, we investigate the role of neonatal HDLs in the regulation of placental vascular function. We show that nHDL is a major carrier of sphingosine-1-phosphate (S1P), which is anchored to the particle through apoM (rs = 0.90, p < 0.0001) in the fetal circulation. Furthermore, this complex interacts with S1P receptors on the feto-placental endothelium and activates specifically extracellular signal-regulated protein kinases 1 and 2 (ERK) and phospholipase C (PLC) downstream signaling, promotes endothelial cell proliferation and calcium flux. Notably, the nHDL-S1P complex triggers actin filaments reorganization, leading to an enhancement of placental endothelial barrier function. Additionally, nHDL induces vasorelaxation of isolated placental chorionic arteries. Taken together, these results suggest that circulating nHDL exerts vasoprotective effects on the feto-placental endothelial barrier mainly via S1P signaling.

KW - Bioactive lipids

KW - High-density lipoprotein

KW - Human placenta

KW - Placental vascular endothelium

KW - Pregnancy

KW - Lípidos bioactivos

KW - Lipoproteína de alta densidad

KW - Placenta humana

KW - Endotelio vascular placentario

KW - El embarazo

UR - http://www.scopus.com/inward/record.url?scp=85078071213&partnerID=8YFLogxK

U2 - 10.1016/j.bbalip.2020.158632

DO - 10.1016/j.bbalip.2020.158632

M3 - Article

C2 - 31954174

AN - SCOPUS:85078071213

VL - 1865

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 4

M1 - 158632

ER -

Circulating cord blood HDL-S1P complex preserves the integrity of the feto-placental vasculature

Resumen

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