Chorion mesenchymal stem cells show superior differentiation, immunosuppressive, and angiogenic potentials in comparison with haploidentical maternal placental cells

Paz L. González, Catalina Carvajal, Jimena Cuenca, Francisca Alcayaga-Miranda, Fernando E. Figueroa, Jorge Bartolucci, Lorena Salazar-Aravena, Maroun Khoury*

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

40 Citas (Scopus)

Resumen

Mesenchymal stem cells (MSCs) of placental origin have become increasingly translational owing to their abundance and accessibility. MSCs of different origin share several features but also present biological differences that might point to distinct clinical properties. Hence, mixing fetal and maternal cells from the same placenta can lead to contradicting results. We analyzed the biological characteristics of haploidentical MSCs isolated from fetal sources, including the umbilical cord (UC-MSCs) and chorion (Ch-MSCs), compared with maternal decidua MSCs (Dc-MSCs). All MSCs were analyzed for general stem cell properties. In addition, immunosuppressive capacity was assessed by the inhibition of T-cell proliferation, and angiogenic potential was evaluated in a Matrigel transplantation assay. The comparison between haploidentical MSCs displayed several distinct features, including (a) marked differences in the expression of CD56, (b) a higher proliferative capacity for Dc-MSCs and UC-MSCs than for Ch-MSCs, (c) a diversity of mesodermal differentiation potential in favor of fetal MSCs, (d) a higher capacity for Ch-MSCs to inhibit T-cell proliferation, and (e) superior angiogenic potential of Ch-MSCs evidenced by a higher capability to form tubular vessel-like structures and an enhanced release of hepatocyte growth factor and vascular endothelial growth factor under hypoxic conditions. Our results suggest that assessing the prevalence of fetomaternal contamination within placental MSCs is necessary to increase robustness and limit side effects in their clinical use. Finally, our work presents evidence positioning fetoplacental cells and notably Ch-MSCs in the forefront of the quest for cell types that are superior for applications in regenerative medicine.
Idioma originalInglés
Páginas (desde-hasta)1109-1121
Número de páginas13
PublicaciónStem cells translational medicine
Volumen4
N.º10
DOI
EstadoPublicada - 1 oct 2015

Nota bibliográfica

Publisher Copyright:
© AlphaMed Press.

Palabras clave

  • Angiogenic potential
  • Chorion
  • Decidua
  • Fetal
  • Immunosuppression
  • Maternal
  • Mesenchymal stem cells
  • Placental cells
  • Umbilical cord

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