Characterization of the leukocyte-and platelet-rich fibrin block: Release of growth factors, cellular content, and structure

Ana B. Castro*, Simone Cortellini, Andy Temmerman, Xin Li, Nelson Pinto, Wim Teughels, Marc Quirynen

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

23 Citas (Scopus)

Resumen

Purpose: The leukocyte- and platelet-rich fibrin block (L-PRF block) is a composite graft that combines a xenograft that is acting as a scaffold with L-PRF membranes that serve as a bioactive nodule with osteoinductive capacity. This study evaluated the properties of the L-PRF block and its components in terms of release of growth factors, cellular content, and structure. Materials and Methods: The concentration of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), platelet-derived growth factor-AB (PDGF-AB), and bone morphogenetic protein-1 (BMP-1) released by an L-PRF membrane and an L-PRF block were examined with ELISA for five time intervals (0 to 4 hours, 4 hours to 1 day, 1 to 3 days, 3 to 7 days, 7 to 14 days). Those levels in L-PRF exudate and liquid fibrinogen were also evaluated. The cellular content of the liquid fibrinogen, L-PRF membrane, and exudate was calculated. The L-PRF block was also analyzed by means of a microcomputed tomography (micro-CT) scan and scanning electron microscopy (SEM). Results: TGF-β1 was the most released growth factor after 14 days, followed by PDGF-AB, VEGF, and BMP-1. All L-PRF blocks constantly released the four growth factors up to 14 days. L-PRF membrane and liquid fibrinogen presented high concentrations of leukocytes and platelets. The micro-CT and SEM images revealed the bone substitute particles surrounded by platelets and leukocytes, embedded in a dense fibrin network. Conclusion: The L-PRF block consists of deproteinized bovine bone mineral particles surrounded by platelets and leukocytes, embedded in a fibrin network that releases growth factors up to 14 days.
Idioma originalInglés
Páginas (desde-hasta)855-864
Número de páginas10
PublicaciónInternational Journal of Oral and Maxillofacial Implants
Volumen34
N.º4
DOI
EstadoPublicada - 2019

Nota bibliográfica

Publisher Copyright:
© 2019 by Quintessence Publishing Co Inc.

Palabras clave

  • Blood platelets
  • Cell count
  • Fibrinogen
  • Growth Factors
  • Scanning electron microscopy
  • Tissue engineering

Huella

Profundice en los temas de investigación de 'Characterization of the leukocyte-and platelet-rich fibrin block: Release of growth factors, cellular content, and structure'. En conjunto forman una huella única.

Citar esto