TY - JOUR
T1 - A universal dental adhesive containing copper nanoparticles stabilizes the hybrid layer in eroded dentin after 1 year
AU - Hanzen, Taíse Alessandra
AU - Gutiérrez, Mario Felipe
AU - de Paris Matos, Thalita
AU - Mara de Paula, Alexandra
AU - Figueredo de Siqueira, Fabiana Suelen
AU - Millán Cardenas, Andrés Felipe
AU - Viteri-Garcia, Andres
AU - Hernandez, Marcela
AU - Loguercio, Alessandro D.
AU - Reis, Alessandra
N1 - Funding Information:
This study was partially supported by the National Council for Scientific and Technological Development (CNPq) , under grants 303332/2017–4 and 305588/2014–1 and the Coordination of Improvement of Higher-Level Personnel ( CAPES ).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2022/3
Y1 - 2022/3
N2 - This study evaluated the matrix metalloproteases (MMP) inhibition of the copper nanoparticles (CuNp), and the effects of their addition into a universal adhesive system on antimicrobial activity (AMA), ultimate tensile strength (UTS), water sorption (WS), solubility (SO), as well as the 24h and 1y microtensile bond strength (μTBS), nanoleakage (NL) and in situ degree of conversion (DC), on sound and eroded dentin. Anti-MMP activity was evaluated for MMP-2, -8 and -9. CuNp (0%[control] and 0.1 wt%) were added into Ambar Universal. Antimicrobial activity against S. mutans was tested using agar diffusion. For UTS, specimens were tested after 24h and 28d. WS and SO were evaluated during 56d. After induced erosion, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks, which were used for evaluation of μTBS and nanoleakage at the 24h and 1y periods. DC was evaluated at the 24h period in these beam-like specimens. Data were submitted to appropriate statistical analyses (α = 0.05). CuNp demonstrated anti MMP-activity (p < 0.05) besides providing antimicrobial activity (p < 0.05), as well as maintained stable μTBS and NL values over 1-year, for both substrates (p < 0.05). Thus, CuNp may prevent the degradation of the adhesive interface, mainly in eroded dentin. Clinical relevance: Copper-containing universal adhesives could be considered a feasible alternative to improve adhesive properties to sound and eroded dentin after 1-year.
AB - This study evaluated the matrix metalloproteases (MMP) inhibition of the copper nanoparticles (CuNp), and the effects of their addition into a universal adhesive system on antimicrobial activity (AMA), ultimate tensile strength (UTS), water sorption (WS), solubility (SO), as well as the 24h and 1y microtensile bond strength (μTBS), nanoleakage (NL) and in situ degree of conversion (DC), on sound and eroded dentin. Anti-MMP activity was evaluated for MMP-2, -8 and -9. CuNp (0%[control] and 0.1 wt%) were added into Ambar Universal. Antimicrobial activity against S. mutans was tested using agar diffusion. For UTS, specimens were tested after 24h and 28d. WS and SO were evaluated during 56d. After induced erosion, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks, which were used for evaluation of μTBS and nanoleakage at the 24h and 1y periods. DC was evaluated at the 24h period in these beam-like specimens. Data were submitted to appropriate statistical analyses (α = 0.05). CuNp demonstrated anti MMP-activity (p < 0.05) besides providing antimicrobial activity (p < 0.05), as well as maintained stable μTBS and NL values over 1-year, for both substrates (p < 0.05). Thus, CuNp may prevent the degradation of the adhesive interface, mainly in eroded dentin. Clinical relevance: Copper-containing universal adhesives could be considered a feasible alternative to improve adhesive properties to sound and eroded dentin after 1-year.
KW - Copper
KW - Degree of conversion
KW - Microtensile bond strength
KW - Nanoleakage
KW - Nanoparticles
KW - Universal adhesive system
UR - http://www.scopus.com/inward/record.url?scp=85120785261&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/645e0faa-75d4-38c2-866e-4c44ee5414f8/
U2 - 10.1016/j.ijadhadh.2021.103041
DO - 10.1016/j.ijadhadh.2021.103041
M3 - Article
AN - SCOPUS:85120785261
SN - 0143-7496
VL - 113
SP - 1
EP - 10
JO - International Journal of Adhesion and Adhesives
JF - International Journal of Adhesion and Adhesives
M1 - 103041
ER -