α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility

Alexis Arcos, Matilde De Paola, Diego Gianetti, Diego Acuña, Zahady D. Velásquez, María Paz Miró, Gabriela Toro, Bryan Hinrichsen, Rosa Iris Muñoz, Yimo Lin, Gonzalo A. Mardones, Pamela Ehrenfeld, Francisco J. Rivera, Marcela A. Michaut*, Luis Federico Batiz

*Autor correspondiente de este trabajo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events. In addition, it regulates cell-to-cell adhesion, AMPK signaling, autophagy and apoptosis in different cell types. In this study we examined the expression pattern of α-SNAP in ovarian tissue and the consequences of α-SNAP (M105I) mutation (hyh mutation) in folliculogenesis and female fertility. Our results showed that α-SNAP protein is highly expressed in GCs and its expression is modulated by gonadotropin stimuli. On the other hand, α-SNAP-mutant mice show a reduction in α-SNAP protein levels. Moreover, increased apoptosis of GCs and follicular atresia, reduced ovulation rate, and a dramatic decline in fertility is observed in α-SNAP-mutant females. In conclusion, α-SNAP plays a critical role in the balance between follicular development and atresia. Consequently, a reduction in its expression/function (M105I mutation) causes early depletion of ovarian follicles and female subfertility.

Idioma originalInglés
Número de artículo11765
PublicaciónScientific Reports
Volumen7
N.º1
DOI
EstadoPublicada - 1 dic 2017

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