Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin

Mario Felipe Gutiérrez; J. Bermudez; Andrés Dávila-Sánchez; Luisa F. Alegría-Acevedo; Luján Méndez-Bauer; Marcela Hernández; Jessica Astorga; Alessandra Reis; Alessandro D. Loguercio; Paulo V. Farago; Eduardo Fernández

doi:10.1016/j.jmbbm.2019.07.024

Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin

Mario Felipe Gutiérrez, J. Bermudez, Andrés Dávila-Sánchez, Luisa F. Alegría-Acevedo, Luján Méndez-Bauer, Marcela Hernández, Jessica Astorga, Alessandra Reis, Alessandro D. Loguercio^*, Paulo V. Farago, Eduardo Fernández

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

This study evaluated the MMP inhibition of the zinc oxide and copper nanoparticles (ZnO/CuNp), and the effects of their addition into adhesives on antimicrobial activity (AMA), ultimate tensile strength (UTS), in vitro degree of conversion (in vitro-DC), as well as, resin–dentin bond strength (μTBS), nanoleakage (NL) and in situ-DC on caries-affected dentin. Anti-MMP activity was evaluated for several MMPs. ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added into Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. UTS were tested after 24 h and 28d. After induced caries, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks. μTBS, NL, in vitro-DC and in situ-DC were evaluated after 24 h. ANOVA and Tukey's test were applied (α = 0.05). ZnO/CuNp demonstrated anti-MMP activity (p < 0.05). The addition of ZnO/CuNp increased AMA and UTS (AMB; p < 0.05). UTS for PBA, in vitro-DC, in situ-DC and μTBS for both adhesives were maintained with ZnO/CuNp (p > 0.05). However, lower NL was observed for ZnO/CuNp groups (p < 0.05). The addition of ZnO/CuNp in adhesives may be an alternative to provide antimicrobial, anti-MMP activities and improves the integrity of the hybrid layer on caries-affected dentin.

Original language	English
Article number	103366
Journal	Journal of the Mechanical Behavior of Biomedical Materials
Volume	100
DOIs	https://doi.org/10.1016/j.jmbbm.2019.07.024
State	Published - Dec 2019
Externally published	Yes

Bibliographical note

Funding Information:
This study was performed by Mario Felipe Gutiérrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Científico y Tecnológico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development ( CNPq ) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 .

Funding Information:
This study was performed by Mario Felipe Guti?rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001.

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Copper
Microtensile bond strength
Nanoleakage
Nanoparticles
Universal adhesive system
Zinc oxide

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10.1016/j.jmbbm.2019.07.024

Cite this

Gutiérrez, M. F., Bermudez, J., Dávila-Sánchez, A., Alegría-Acevedo, L. F., Méndez-Bauer, L., Hernández, M., Astorga, J., Reis, A., Loguercio, A. D., Farago, P. V., & Fernández, E. (2019). Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin. Journal of the Mechanical Behavior of Biomedical Materials, 100, Article 103366. https://doi.org/10.1016/j.jmbbm.2019.07.024

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title = "Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin",

abstract = "This study evaluated the MMP inhibition of the zinc oxide and copper nanoparticles (ZnO/CuNp), and the effects of their addition into adhesives on antimicrobial activity (AMA), ultimate tensile strength (UTS), in vitro degree of conversion (in vitro-DC), as well as, resin–dentin bond strength (μTBS), nanoleakage (NL) and in situ-DC on caries-affected dentin. Anti-MMP activity was evaluated for several MMPs. ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added into Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. UTS were tested after 24 h and 28d. After induced caries, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks. μTBS, NL, in vitro-DC and in situ-DC were evaluated after 24 h. ANOVA and Tukey's test were applied (α = 0.05). ZnO/CuNp demonstrated anti-MMP activity (p < 0.05). The addition of ZnO/CuNp increased AMA and UTS (AMB; p < 0.05). UTS for PBA, in vitro-DC, in situ-DC and μTBS for both adhesives were maintained with ZnO/CuNp (p > 0.05). However, lower NL was observed for ZnO/CuNp groups (p < 0.05). The addition of ZnO/CuNp in adhesives may be an alternative to provide antimicrobial, anti-MMP activities and improves the integrity of the hybrid layer on caries-affected dentin.",

keywords = "Copper, Microtensile bond strength, Nanoleakage, Nanoparticles, Universal adhesive system, Zinc oxide",

author = "Guti{\'e}rrez, {Mario Felipe} and J. Bermudez and Andr{\'e}s D{\'a}vila-S{\'a}nchez and Alegr{\'i}a-Acevedo, {Luisa F.} and Luj{\'a}n M{\'e}ndez-Bauer and Marcela Hern{\'a}ndez and Jessica Astorga and Alessandra Reis and Loguercio, {Alessandro D.} and Farago, {Paulo V.} and Eduardo Fern{\'a}ndez",

note = "Funding Information: This study was performed by Mario Felipe Guti{\'e}rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient{\'i}fico y Tecnol{\'o}gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development ( CNPq ) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior - Brasil (CAPES) - Finance Code 001 . Funding Information: This study was performed by Mario Felipe Guti?rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = dec,

doi = "10.1016/j.jmbbm.2019.07.024",

language = "English",

volume = "100",

journal = "Journal of the Mechanical Behavior of Biomedical Materials",

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Gutiérrez, MF, Bermudez, J, Dávila-Sánchez, A, Alegría-Acevedo, LF, Méndez-Bauer, L, Hernández, M, Astorga, J, Reis, A, Loguercio, AD, Farago, PV & Fernández, E 2019, 'Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin', Journal of the Mechanical Behavior of Biomedical Materials, vol. 100, 103366. https://doi.org/10.1016/j.jmbbm.2019.07.024

TY - JOUR

T1 - Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin

AU - Gutiérrez, Mario Felipe

AU - Bermudez, J.

AU - Dávila-Sánchez, Andrés

AU - Alegría-Acevedo, Luisa F.

AU - Méndez-Bauer, Luján

AU - Hernández, Marcela

AU - Astorga, Jessica

AU - Reis, Alessandra

AU - Loguercio, Alessandro D.

AU - Farago, Paulo V.

AU - Fernández, Eduardo

N1 - Funding Information: This study was performed by Mario Felipe Gutiérrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Científico y Tecnológico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development ( CNPq ) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 . Funding Information: This study was performed by Mario Felipe Guti?rrez Reyes as partial fulfillment of his PhD degree at the State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil. This project was supported by Fondecyt (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico - Chile) project 1170575 (Chile; EF). Also, this study was partially supported by the National Council for Scientific and Technological Development (CNPq) under grants 305588/2014-1 (Brazil; ADL) and in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001. Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/12

Y1 - 2019/12

N2 - This study evaluated the MMP inhibition of the zinc oxide and copper nanoparticles (ZnO/CuNp), and the effects of their addition into adhesives on antimicrobial activity (AMA), ultimate tensile strength (UTS), in vitro degree of conversion (in vitro-DC), as well as, resin–dentin bond strength (μTBS), nanoleakage (NL) and in situ-DC on caries-affected dentin. Anti-MMP activity was evaluated for several MMPs. ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added into Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. UTS were tested after 24 h and 28d. After induced caries, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks. μTBS, NL, in vitro-DC and in situ-DC were evaluated after 24 h. ANOVA and Tukey's test were applied (α = 0.05). ZnO/CuNp demonstrated anti-MMP activity (p < 0.05). The addition of ZnO/CuNp increased AMA and UTS (AMB; p < 0.05). UTS for PBA, in vitro-DC, in situ-DC and μTBS for both adhesives were maintained with ZnO/CuNp (p > 0.05). However, lower NL was observed for ZnO/CuNp groups (p < 0.05). The addition of ZnO/CuNp in adhesives may be an alternative to provide antimicrobial, anti-MMP activities and improves the integrity of the hybrid layer on caries-affected dentin.

AB - This study evaluated the MMP inhibition of the zinc oxide and copper nanoparticles (ZnO/CuNp), and the effects of their addition into adhesives on antimicrobial activity (AMA), ultimate tensile strength (UTS), in vitro degree of conversion (in vitro-DC), as well as, resin–dentin bond strength (μTBS), nanoleakage (NL) and in situ-DC on caries-affected dentin. Anti-MMP activity was evaluated for several MMPs. ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added into Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. UTS were tested after 24 h and 28d. After induced caries, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin–dentin sticks. μTBS, NL, in vitro-DC and in situ-DC were evaluated after 24 h. ANOVA and Tukey's test were applied (α = 0.05). ZnO/CuNp demonstrated anti-MMP activity (p < 0.05). The addition of ZnO/CuNp increased AMA and UTS (AMB; p < 0.05). UTS for PBA, in vitro-DC, in situ-DC and μTBS for both adhesives were maintained with ZnO/CuNp (p > 0.05). However, lower NL was observed for ZnO/CuNp groups (p < 0.05). The addition of ZnO/CuNp in adhesives may be an alternative to provide antimicrobial, anti-MMP activities and improves the integrity of the hybrid layer on caries-affected dentin.

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KW - Microtensile bond strength

KW - Nanoleakage

KW - Nanoparticles

KW - Universal adhesive system

KW - Zinc oxide

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DO - 10.1016/j.jmbbm.2019.07.024

M3 - Article

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AN - SCOPUS:85070626363

SN - 1751-6161

VL - 100

JO - Journal of the Mechanical Behavior of Biomedical Materials

JF - Journal of the Mechanical Behavior of Biomedical Materials

M1 - 103366

ER -

Zinc oxide and copper nanoparticles addition in universal adhesive systems improve interface stability on caries-affected dentin

Abstract

Bibliographical note

Keywords

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