@inbook{3f52ef53c14541259da515a21d026295,
title = "Two-dimensional gel electrophoresis-based proteomic analysis of brain synapses",
abstract = "Neuroproteomic technologies are crucial for a deeper understanding of molecular mechanisms underlying neuronal plasticity and pathologies of the central nervous system. For a comprehensive neuroproteomic analysis, high-resolution high-throughput techniques are indispensable. The most commonly used-system for 2D gel electrophoresis is based on the combination of isoelectric focussing and sodium dodecyl-sulphate-polyacrylamide gel electrophoresis. Nevertheless, the analysis of complex samples derived from biochemically prepared synaptic fractions raises some crucial challenges. Therefore, we describe the-general 2D gel electrophoresis procedure, including sample preparation, gel casting, electrophoresis-condition, and the detection of proteins on the gel with mass spectrometry compatible silver staining, sypro-ruby or colloidal Coomassie Brilliant Blue.",
keywords = "2D gel electrophoresis, Image analysis, Isoelectric focussing, Protein staining, SDS-PAGE, Sample preparation",
author = "Smalla, {Karl Heinz} and Ursula Wyneken",
year = "2011",
doi = "10.1007/978-1-61779-111-6_8",
language = "English",
isbn = "9781617791109",
series = "Neuromethods",
pages = "95--113",
editor = "Li, {Ka Wan}",
booktitle = "Neuroproteomics",
}
