Two-dimensional gel electrophoresis-based proteomic analysis of brain synapses

Karl Heinz Smalla*, Ursula Wyneken

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


Neuroproteomic technologies are crucial for a deeper understanding of molecular mechanisms underlying neuronal plasticity and pathologies of the central nervous system. For a comprehensive neuroproteomic analysis, high-resolution high-throughput techniques are indispensable. The most commonly used-system for 2D gel electrophoresis is based on the combination of isoelectric focussing and sodium dodecyl-sulphate-polyacrylamide gel electrophoresis. Nevertheless, the analysis of complex samples derived from biochemically prepared synaptic fractions raises some crucial challenges. Therefore, we describe the-general 2D gel electrophoresis procedure, including sample preparation, gel casting, electrophoresis-condition, and the detection of proteins on the gel with mass spectrometry compatible silver staining, sypro-ruby or colloidal Coomassie Brilliant Blue.

Original languageEnglish
Title of host publicationNeuroproteomics
EditorsKa Wan Li
Number of pages19
StatePublished - 2011
Externally publishedYes

Publication series

ISSN (Print)0893-2336
ISSN (Electronic)1940-6045


  • 2D gel electrophoresis
  • Image analysis
  • Isoelectric focussing
  • Protein staining
  • Sample preparation


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