Tuning a P450 enzyme for methane oxidation

Felipe E. Zilly; Juan Pablo Acevedo; Wojciech Augustyniak; Alfred Deege; Manfred T. Reetz

doi:10.1002/anie.201006587

Tuning a P450 enzyme for methane oxidation

Felipe E. Zilly, Juan Pablo Acevedo, Wojciech Augustyniak, Alfred Deege, Manfred T. Reetz

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

A new spin: The addition of chemically inert perfluoro carboxylic acids (green; see picture) to P450 enzymes results in dramatic activation of their catalytic activity as a result of the conversion of the Fe/heme from a low-spin to a high-spin state, and the reduction of the binding-pocket size. Together these effects allow otherwise inert substrates such as propane and even methane to be oxidized.

Original language	English
Pages (from-to)	2720-2724
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	12
DOIs	https://doi.org/10.1002/anie.201006587
State	Published - 14 Mar 2011
Externally published	Yes

Keywords

alkanes
C-H activation
host-guest systems
methane oxidation
P450 enzymes

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10.1002/anie.201006587

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abstract = "A new spin: The addition of chemically inert perfluoro carboxylic acids (green; see picture) to P450 enzymes results in dramatic activation of their catalytic activity as a result of the conversion of the Fe/heme from a low-spin to a high-spin state, and the reduction of the binding-pocket size. Together these effects allow otherwise inert substrates such as propane and even methane to be oxidized.",

keywords = "alkanes, C-H activation, host-guest systems, methane oxidation, P450 enzymes",

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AU - Zilly, Felipe E.

AU - Acevedo, Juan Pablo

AU - Augustyniak, Wojciech

AU - Deege, Alfred

AU - Reetz, Manfred T.

PY - 2011/3/14

Y1 - 2011/3/14

N2 - A new spin: The addition of chemically inert perfluoro carboxylic acids (green; see picture) to P450 enzymes results in dramatic activation of their catalytic activity as a result of the conversion of the Fe/heme from a low-spin to a high-spin state, and the reduction of the binding-pocket size. Together these effects allow otherwise inert substrates such as propane and even methane to be oxidized.

AB - A new spin: The addition of chemically inert perfluoro carboxylic acids (green; see picture) to P450 enzymes results in dramatic activation of their catalytic activity as a result of the conversion of the Fe/heme from a low-spin to a high-spin state, and the reduction of the binding-pocket size. Together these effects allow otherwise inert substrates such as propane and even methane to be oxidized.

KW - alkanes

KW - C-H activation

KW - host-guest systems

KW - methane oxidation

KW - P450 enzymes

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M3 - Article

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Tuning a P450 enzyme for methane oxidation

Abstract

Keywords

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