Tuning a P450 enzyme for methane oxidation

Felipe E. Zilly, Juan P. Acevedo, Wojciech Augustyniak, Alfred Deege, Manfred T. Reetz

Research output: Contribution to journalArticlepeer-review

126 Scopus citations


A new spin: The addition of chemically inert perfluoro carboxylic acids (green; see picture) to P450 enzymes results in dramatic activation of their catalytic activity as a result of the conversion of the Fe/heme from a low-spin to a high-spin state, and the reduction of the binding-pocket size. Together these effects allow otherwise inert substrates such as propane and even methane to be oxidized.

Original languageEnglish
Pages (from-to)2720-2724
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number12
StatePublished - 14 Mar 2011
Externally publishedYes


  • C-H activation
  • P450 enzymes
  • alkanes
  • host-guest systems
  • methane oxidation


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