Towards dense motion estimation in light and electron microscopy

Luis Pizarro, José Francisco Delpiano, Paul Aljabar, Javier Ruiz-Del-Solar, Daniel Rueckert

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

8 Scopus citations

Abstract

Motion estimation, also known as optic flow, refers to the process of determining a 2D displacement field that aligns two images. Most methods that estimate motion or deformation fields in biological image sequences rely on sparse, distinct features (landmarks). Going a step forward, we are interested in methods to compute dense deformation fields (for all pixels). In this paper we compare two of such frameworks: the B-splines based free-form deformation (FFD) approach, which is well-known in medical image registration; and the combined local-global (CLG) approach, a popular optic flow method in computer vision. We test both methods on synthetic and real image sequences obtained by confocal light microscopy and by scanning electron microscopy, showing their performance in terms of accuracy and computational cost. As an alternative to traditional sparse techniques, the estimation of dense motion fields would allow tackling other related problems with sub-pixel precision, for example, the segmentation and classification of different biological structures according to their local motion, trajectory, growth and development.

Original languageEnglish
Title of host publication2011 8th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI'11
Pages1939-1942
Number of pages4
DOIs
StatePublished - 2011
Externally publishedYes
Event2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11 - Chicago, IL, United States
Duration: 30 Mar 20112 Apr 2011

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Conference

Conference2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11
Country/TerritoryUnited States
CityChicago, IL
Period30/03/112/04/11

Keywords

  • deformation field
  • electron microscopy
  • light microscopy
  • Motion estimation
  • optic flow

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