TY - JOUR
T1 - The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes
AU - Aldana, Catherine Andrade
AU - Amenabar, Felipe Ugarte
AU - Silva, Carolina Inostroza
AU - Calderon, Paulo Diaz
AU - Messina, David Rosenberg
AU - Carrasco, Nelson Pinto
AU - Quirynen, Marc
N1 - Publisher Copyright:
© 2022
PY - 2022/11/1
Y1 - 2022/11/1
N2 - Aim: The purpose of this study was to evaluate the impact of gender and peripheral blood parameters on the characteristics of Leucocyte-and Platelet-Rich Fibrin (L-PRF) membranes and to describe histologically three different zones of L-PRF membranes.Methods: Blood was collected from twenty healthy donors (10 men and 10 women). Peripheral blood parameters including leucocyte and platelet counts, and fibrinogen levels were recorded. L-PRF membranes were prepared to quantify the release of growth factors (PDGF, VEGF, BMP-2, and BMP-9) at 1, 2, 3 and 7 days and for histological examination. Three zones within each L-PRF membrane (face, body, and tail) were analysed separately, quantifying the area of leucocytes, platelets, and fibrin in percentage. The Young's modulus of the membranes was also considered (during tensile and compression tests).Results: Women had significantly higher fibrinogen levels in their peripheral blood, and a higher release of BMP-9, whereas men showed a significantly higher Young's modulus in compression tests. The histology revealed significant differences in cellular content and fibrin concentration between the 3 areas, with the face being biologically the richest.Conclusion: Several factors influenced the final characteristics of L-PRF membranes. These need to be taken into consideration when interpreting the results of research, but especially in clinical practice.
AB - Aim: The purpose of this study was to evaluate the impact of gender and peripheral blood parameters on the characteristics of Leucocyte-and Platelet-Rich Fibrin (L-PRF) membranes and to describe histologically three different zones of L-PRF membranes.Methods: Blood was collected from twenty healthy donors (10 men and 10 women). Peripheral blood parameters including leucocyte and platelet counts, and fibrinogen levels were recorded. L-PRF membranes were prepared to quantify the release of growth factors (PDGF, VEGF, BMP-2, and BMP-9) at 1, 2, 3 and 7 days and for histological examination. Three zones within each L-PRF membrane (face, body, and tail) were analysed separately, quantifying the area of leucocytes, platelets, and fibrin in percentage. The Young's modulus of the membranes was also considered (during tensile and compression tests).Results: Women had significantly higher fibrinogen levels in their peripheral blood, and a higher release of BMP-9, whereas men showed a significantly higher Young's modulus in compression tests. The histology revealed significant differences in cellular content and fibrin concentration between the 3 areas, with the face being biologically the richest.Conclusion: Several factors influenced the final characteristics of L-PRF membranes. These need to be taken into consideration when interpreting the results of research, but especially in clinical practice.
KW - Fibrinogen
KW - Growth factors
KW - Histology
KW - L-PRF
KW - Leucocytes
KW - Platelet concentrates
KW - Platelet rich fibrin
UR - http://www.scopus.com/inward/record.url?scp=85137703596&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/0a891297-ddfa-37f1-80af-5d26b42993ba/
U2 - 10.1016/j.jobcr.2022.08.020
DO - 10.1016/j.jobcr.2022.08.020
M3 - Article
C2 - 36118143
AN - SCOPUS:85137703596
SN - 2212-4268
VL - 12
SP - 753
EP - 759
JO - Journal of Oral Biology and Craniofacial Research
JF - Journal of Oral Biology and Craniofacial Research
IS - 6
ER -