The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus

Claudio Pérez, Fermín E. González, Violeta Pavez, Aida V. Araya, Adam Aguirre, Andrea Cruzat, Juan Contreras-Levicoy, Andrés Dotte, Octavio Aravena, Lorena Salazar, Diego Catalán, Jimena Cuenca, Arturo Ferreira, Irene Schiattino, Juan C. Aguillón

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-α gene promoter. The transition G→A at position -308 generates the TNF-α1 (G/G) and TNF-α2 (G/A or A/A) alleles, where the polymorphic TNF-α2 allele is associated with a high, in vitro TNF-α expression and an increased susceptibility to diverse illnesses. Here we study the association of the -308 TNF-α SNP with the susceptibility for developing aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the TNF-α capability expression and the presence of the -308 polymorphism. For this purpose we recruited 27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and 27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group was comprised of 30 subjects. Genotyping for TNF-α promoter was performed by PCR-RFLP analysis. For TNF-α expression we used a blood culture system.

Original languageEnglish
Pages (from-to)364-370
Number of pages7
JournalEuropean Cytokine Network
Volume15
Issue number4
StatePublished - Oct 2004
Externally publishedYes

Keywords

  • Cytokine
  • Diabetes
  • Periodontitis
  • Polymorphism
  • Promoter
  • TNF-α gene promoter
  • Tumor necrosis factor
  • Type 1 diabetes mellitus

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