The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus

Claudio Pérez; Fermín E. González; Violeta Pavez; Aida V. Araya; Adam Aguirre; Andrea Cruzat; Juan Contreras-Levicoy; Andrés Dotte; Octavio Aravena; Lorena Salazar; Diego Catalán; Jimena Cuenca; Arturo Ferreira; Irene Schiattino; Juan C. Aguillón

The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus

Claudio Pérez, Fermín E. González, Violeta Pavez, Aida V. Araya, Adam Aguirre, Andrea Cruzat, Juan Contreras-Levicoy, Andrés Dotte, Octavio Aravena, Lorena Salazar, Diego Catalán, Jimena Cuenca, Arturo Ferreira, Irene Schiattino, Juan C. Aguillón^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-α gene promoter. The transition G→A at position -308 generates the TNF-α1 (G/G) and TNF-α2 (G/A or A/A) alleles, where the polymorphic TNF-α2 allele is associated with a high, in vitro TNF-α expression and an increased susceptibility to diverse illnesses. Here we study the association of the -308 TNF-α SNP with the susceptibility for developing aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the TNF-α capability expression and the presence of the -308 polymorphism. For this purpose we recruited 27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and 27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group was comprised of 30 subjects. Genotyping for TNF-α promoter was performed by PCR-RFLP analysis. For TNF-α expression we used a blood culture system.

Original language	English
Pages (from-to)	364-370
Number of pages	7
Journal	European Cytokine Network
Volume	15
Issue number	4
State	Published - Oct 2004
Externally published	Yes

Keywords

Cytokine
Diabetes
Periodontitis
Polymorphism
Promoter
TNF-α gene promoter
Tumor necrosis factor
Type 1 diabetes mellitus

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Pérez, C., González, F. E., Pavez, V., Araya, A. V., Aguirre, A., Cruzat, A., Contreras-Levicoy, J., Dotte, A., Aravena, O., Salazar, L., Catalán, D., Cuenca, J., Ferreira, A., Schiattino, I., & Aguillón, J. C. (2004). The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus. European Cytokine Network, 15(4), 364-370.

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title = "The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus",

abstract = "Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-α gene promoter. The transition G→A at position -308 generates the TNF-α1 (G/G) and TNF-α2 (G/A or A/A) alleles, where the polymorphic TNF-α2 allele is associated with a high, in vitro TNF-α expression and an increased susceptibility to diverse illnesses. Here we study the association of the -308 TNF-α SNP with the susceptibility for developing aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the TNF-α capability expression and the presence of the -308 polymorphism. For this purpose we recruited 27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and 27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group was comprised of 30 subjects. Genotyping for TNF-α promoter was performed by PCR-RFLP analysis. For TNF-α expression we used a blood culture system.",

keywords = "Cytokine, Diabetes, Periodontitis, Polymorphism, Promoter, TNF-α gene promoter, Tumor necrosis factor, Type 1 diabetes mellitus",

author = "Claudio P{\'e}rez and Gonz{\'a}lez, {Ferm{\'i}n E.} and Violeta Pavez and Araya, {Aida V.} and Adam Aguirre and Andrea Cruzat and Juan Contreras-Levicoy and Andr{\'e}s Dotte and Octavio Aravena and Lorena Salazar and Diego Catal{\'a}n and Jimena Cuenca and Arturo Ferreira and Irene Schiattino and Aguill{\'o}n, {Juan C.}",

year = "2004",

month = oct,

language = "English",

volume = "15",

pages = "364--370",

journal = "European Cytokine Network",

issn = "1148-5493",

publisher = "John Libbey Eurotext",

number = "4",

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Pérez, C, González, FE, Pavez, V, Araya, AV, Aguirre, A, Cruzat, A, Contreras-Levicoy, J, Dotte, A, Aravena, O, Salazar, L, Catalán, D, Cuenca, J, Ferreira, A, Schiattino, I & Aguillón, JC 2004, 'The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus', European Cytokine Network, vol. 15, no. 4, pp. 364-370.

The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus. / Pérez, Claudio; González, Fermín E.; Pavez, Violeta et al.
In: European Cytokine Network, Vol. 15, No. 4, 10.2004, p. 364-370.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus

AU - Pérez, Claudio

AU - González, Fermín E.

AU - Pavez, Violeta

AU - Araya, Aida V.

AU - Aguirre, Adam

AU - Cruzat, Andrea

AU - Contreras-Levicoy, Juan

AU - Dotte, Andrés

AU - Aravena, Octavio

AU - Salazar, Lorena

AU - Catalán, Diego

AU - Cuenca, Jimena

AU - Ferreira, Arturo

AU - Schiattino, Irene

AU - Aguillón, Juan C.

PY - 2004/10

Y1 - 2004/10

N2 - Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-α gene promoter. The transition G→A at position -308 generates the TNF-α1 (G/G) and TNF-α2 (G/A or A/A) alleles, where the polymorphic TNF-α2 allele is associated with a high, in vitro TNF-α expression and an increased susceptibility to diverse illnesses. Here we study the association of the -308 TNF-α SNP with the susceptibility for developing aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the TNF-α capability expression and the presence of the -308 polymorphism. For this purpose we recruited 27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and 27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group was comprised of 30 subjects. Genotyping for TNF-α promoter was performed by PCR-RFLP analysis. For TNF-α expression we used a blood culture system.

AB - Several single-nucleotide polymorphisms (SNPs) have been identified in the TNF-α gene promoter. The transition G→A at position -308 generates the TNF-α1 (G/G) and TNF-α2 (G/A or A/A) alleles, where the polymorphic TNF-α2 allele is associated with a high, in vitro TNF-α expression and an increased susceptibility to diverse illnesses. Here we study the association of the -308 TNF-α SNP with the susceptibility for developing aggressive periodontitis (AP), AP combined with type 1 diabetes mellitus (DM) and DM. We also explore the TNF-α capability expression and the presence of the -308 polymorphism. For this purpose we recruited 27 individuals with AP (AP+ group), 27 individuals with AP combined with DM (AP+/DM+ group), and 27 individuals with DM without signs of periodontitis upon clinical examination (DM+ group). The control group was comprised of 30 subjects. Genotyping for TNF-α promoter was performed by PCR-RFLP analysis. For TNF-α expression we used a blood culture system.

KW - Cytokine

KW - Diabetes

KW - Periodontitis

KW - Polymorphism

KW - Promoter

KW - TNF-α gene promoter

KW - Tumor necrosis factor

KW - Type 1 diabetes mellitus

UR - http://www.scopus.com/inward/record.url?scp=19944433491&partnerID=8YFLogxK

M3 - Article

C2 - 15627647

AN - SCOPUS:19944433491

SN - 1148-5493

VL - 15

SP - 364

EP - 370

JO - European Cytokine Network

JF - European Cytokine Network

IS - 4

ER -

The -308 polymorphism in the promoter region of the tumor necrosis factor-alpha (TNF-α) gene and ex vivo lipopolysaccharide-induced TNF-α expression in patients with aggressive periodontitis and/or type 1 diabetes mellitus

Abstract

Keywords

UN SDGs

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