TGFβ3 secretion by three-dimensional cultures of human dental apical papilla mesenchymal stem cells

Rodrigo A. Somoza*, Cristian A. Acevedo, Fernando Albornoz, Patricia Luz-Crawford, Flavio Carrión, Manuel E. Young, Caroline Weinstein-Oppenheimer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Mesenchymal stem cells (MSCs) can be isolated from dental tissues, such as pulp and periodontal ligament; the dental apical papilla (DAP) is a less-studied MSC source. These dental-derived MSCs are of great interest because of their potential as an accessible source for cell-based therapies and tissue-engineering (TE) approaches. Much of the interest regarding MSCs relies on the trophic-mediated repair and regenerative effects observed when they are implanted. TGFβ3 is a key growth factor involved in tissue regeneration and scarless tissue repair. We hypothesized that human DAP-derived MSCs (hSCAPs) can produce and secrete TGFβ3 in response to micro-environmental cues. For this, we encapsulated hSCAPs in different types of matrix and evaluated TGFβ3 secretion. We found that dynamic changes of cell–matrix interactions and mechanical stress that cells sense during the transition from a monolayer culture (two-dimensional, 2D) towards a three-dimensional (3D) culture condition, rather than the different chemical composition of the scaffolds, may trigger the TGFβ3 secretion, while monolayer cultures showed almost 10-fold less secretion of TGFβ3. The study of these interactions is provided as a cornerstone in designing future strategies in TE and cell therapy that are more efficient and effective for repair/regeneration of damaged tissues.

Original languageEnglish
Pages (from-to)1045-1056
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number4
StatePublished - 1 Apr 2017

Bibliographical note

Publisher Copyright:
Copyright © 2015 John Wiley & Sons, Ltd.


  • 3D culture
  • SCAP
  • TGFβ3
  • alginate
  • fibrin
  • mesenchymal stem cells


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