Telomerase expression abrogates rapamycin-induced irreversible growth arrest of uterine fibroid smooth muscle cells

Guangli Suo, Anil Sadarangani, Wingchung Tang, Bryan D. Cowan, Jean Y.J. Wang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Uterine fibroids are the most common solid tumors found in women of reproductive age. It has been reported that deregulation of the mammalian target of rapamycin (mTOR) pathway plays an important role in the etiology of leiomyoma. Here, we investigated the effect of rapamycin, an inhibitor of mTORC1, on the growth of primary fibroid smooth muscle cells (fSMCs) and human telomerase reverse transcriptase (hTERT)-transduced and immortalized fSMCs. With the primary fSMCs, a 24-hour treatment with rapamycin was sufficient to trigger a growth arrest that was not reversible upon drug removal. By contrast, the growth inhibitory effect of rapamycin on the hTERT-transduced fSMCs was readily reversible, as these cells resumed proliferation upon the withdrawal of the drug. These results suggest that rapamycin-induced irreversible growth arrest of fSMCs is dependent on the senescence barrier that is abrogated by the ectopic expression of telomerase.

Original languageEnglish
Pages (from-to)1161-1170
Number of pages10
JournalReproductive Sciences
Volume21
Issue number9
DOIs
StatePublished - 1 Sep 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© The Author(s) 2014.

Keywords

  • leiomyoma
  • rapamycin
  • telomerase

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