Synthesis of nanostructured porous silica coatings on titanium and their cell adhesive and osteogenic differentiation properties

Débora Inzunza, Cristian Covarrubias*, Alfredo Von Marttens, Yerko Leighton, Juan Carlos Carvajal, Francisco Valenzuela, Mario Díaz-Dosque, Nicolás Méndez, Constanza Martínez, Ana María Pino, Juan Pablo Rodríguez, Mónica Cáceres, Patricio Smith

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Nanostructured porous silica coatings were synthesized on titanium by the combined sol-gel and evaporation-induced self-assembly process. The silica-coating structures were characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and nitrogen sorptometry. The effect of the nanoporous surface on apatite formation in simulated body fluid, protein adsorption, osteoblast cell adhesion behavior, and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) is reported. Silica coatings with highly ordered sub-10 nm porosity accelerate early osteoblast adhesive response, a favorable cell response that is attributed to an indirect effect due to the high protein adsorption observed on the large-specific surface area of the nanoporous coating but is also probably due to direct mechanical stimulus from the nanostructured topography. The nanoporous silica coatings, particularly those doped with calcium and phosphate, also promote the osteogenic differentiation of hBMSCs with spontaneous mineral nodule formation in basal conditions. The bioactive surface properties exhibited by the nanostructured porous silica coatings make these materials a promising alternative to improve the osseointegration properties of titanium dental implants and could have future impact on the nanoscale design of implant surfaces.

Original languageEnglish
Pages (from-to)37-48
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Issue number1
StatePublished - Jan 2014
Externally publishedYes


  • cell adhesion
  • nanotopography
  • osseointegration
  • sol-gel technique
  • titanium


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