Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds

Cristian A. Acevedo; Elizabeth Sánchez; Paulo Díaz-Calderón; Jonny J. Blaker; Javier Enrione; Franck Quero

doi:10.1002/app.44772

Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds

Cristian A. Acevedo, Elizabeth Sánchez, Paulo Díaz-Calderón, Jonny J. Blaker, Javier Enrione, Franck Quero^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

In this study, synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal, and sorption properties of porous chitosan/gelatin/hyaluronic acid hybrid foams are reported. Fourier transform infrared spectroscopy has been utilized to confirm the covalent attachment of hyaluronic acid to gelatin and chitosan, and covalent chemical crosslinking between gelatin and chitosan. Detailed image analysis of scanning electron microscopy images of the porous scaffold hydrids reveal that the pore size of the materials formulated using either low- or high-molecular-weight chitosan increases significantly upon crosslinking using ethyl(dimethylaminopropyl) carbodiimide/N-Hydroxysuccinimide. These microstructural changes are even more pronounced for the crosslinked hybrid scaffolds formulated using low-molecular-weight chitosan, highlighting a synergistic effect between crosslinking and the use of low-molecular-weight chitosan. Results obtained using differential scanning calorimetry demonstrate a significant reduction in molecular mobility reduction in molecular mobility for crosslinked scaffolds formed using high-molecular-weight chitosan compared to non-crosslinked hybrids and crosslinked hybrids formulated using low-molecular-weight chitosan. Correspondingly, dynamic vapor sorption evidenced significantly lower water vapor sorption for crosslinked scaffolds formulated using high-molecular-weight chitosan.

Original language	English
Article number	44772
Journal	Journal of Applied Polymer Science
Volume	134
Issue number	18
DOIs	https://doi.org/10.1002/app.44772
State	Published - 10 May 2017

Bibliographical note

Funding Information:
The authors gratefully acknowledge financial support from Conicyt Programa PCI/Newton Picarte Grant N? 140144. J. E., C. A. and F.Q. acknowledge, respectively, financial support from Fondecyt under the Regular Grants N? 1140132 and N? 1160311, and the Postdoctoral Grant N? 3140036.

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.

Keywords

biopolymers
chitosan
crosslinking
hybrid
molecular weight
scaffold

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Acevedo, C. A., Sánchez, E., Díaz-Calderón, P., Blaker, J. J., Enrione, J., & Quero, F. (2017). Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds. Journal of Applied Polymer Science, 134(18), Article 44772. https://doi.org/10.1002/app.44772

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title = "Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds",

abstract = "In this study, synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal, and sorption properties of porous chitosan/gelatin/hyaluronic acid hybrid foams are reported. Fourier transform infrared spectroscopy has been utilized to confirm the covalent attachment of hyaluronic acid to gelatin and chitosan, and covalent chemical crosslinking between gelatin and chitosan. Detailed image analysis of scanning electron microscopy images of the porous scaffold hydrids reveal that the pore size of the materials formulated using either low- or high-molecular-weight chitosan increases significantly upon crosslinking using ethyl(dimethylaminopropyl) carbodiimide/N-Hydroxysuccinimide. These microstructural changes are even more pronounced for the crosslinked hybrid scaffolds formulated using low-molecular-weight chitosan, highlighting a synergistic effect between crosslinking and the use of low-molecular-weight chitosan. Results obtained using differential scanning calorimetry demonstrate a significant reduction in molecular mobility reduction in molecular mobility for crosslinked scaffolds formed using high-molecular-weight chitosan compared to non-crosslinked hybrids and crosslinked hybrids formulated using low-molecular-weight chitosan. Correspondingly, dynamic vapor sorption evidenced significantly lower water vapor sorption for crosslinked scaffolds formulated using high-molecular-weight chitosan.",

keywords = "biopolymers, chitosan, crosslinking, hybrid, molecular weight, scaffold",

author = "Acevedo, {Cristian A.} and Elizabeth S{\'a}nchez and Paulo D{\'i}az-Calder{\'o}n and Blaker, {Jonny J.} and Javier Enrione and Franck Quero",

note = "Funding Information: The authors gratefully acknowledge financial support from Conicyt Programa PCI/Newton Picarte Grant N? 140144. J. E., C. A. and F.Q. acknowledge, respectively, financial support from Fondecyt under the Regular Grants N? 1140132 and N? 1160311, and the Postdoctoral Grant N? 3140036. Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",

year = "2017",

month = may,

day = "10",

doi = "10.1002/app.44772",

language = "English",

volume = "134",

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Acevedo, CA, Sánchez, E, Díaz-Calderón, P, Blaker, JJ, Enrione, J & Quero, F 2017, 'Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds', Journal of Applied Polymer Science, vol. 134, no. 18, 44772. https://doi.org/10.1002/app.44772

Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds. / Acevedo, Cristian A.; Sánchez, Elizabeth; Díaz-Calderón, Paulo et al.
In: Journal of Applied Polymer Science, Vol. 134, No. 18, 44772, 10.05.2017.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds

AU - Acevedo, Cristian A.

AU - Sánchez, Elizabeth

AU - Díaz-Calderón, Paulo

AU - Blaker, Jonny J.

AU - Enrione, Javier

AU - Quero, Franck

N1 - Funding Information: The authors gratefully acknowledge financial support from Conicyt Programa PCI/Newton Picarte Grant N? 140144. J. E., C. A. and F.Q. acknowledge, respectively, financial support from Fondecyt under the Regular Grants N? 1140132 and N? 1160311, and the Postdoctoral Grant N? 3140036. Publisher Copyright: © 2017 Wiley Periodicals, Inc.

PY - 2017/5/10

Y1 - 2017/5/10

N2 - In this study, synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal, and sorption properties of porous chitosan/gelatin/hyaluronic acid hybrid foams are reported. Fourier transform infrared spectroscopy has been utilized to confirm the covalent attachment of hyaluronic acid to gelatin and chitosan, and covalent chemical crosslinking between gelatin and chitosan. Detailed image analysis of scanning electron microscopy images of the porous scaffold hydrids reveal that the pore size of the materials formulated using either low- or high-molecular-weight chitosan increases significantly upon crosslinking using ethyl(dimethylaminopropyl) carbodiimide/N-Hydroxysuccinimide. These microstructural changes are even more pronounced for the crosslinked hybrid scaffolds formulated using low-molecular-weight chitosan, highlighting a synergistic effect between crosslinking and the use of low-molecular-weight chitosan. Results obtained using differential scanning calorimetry demonstrate a significant reduction in molecular mobility reduction in molecular mobility for crosslinked scaffolds formed using high-molecular-weight chitosan compared to non-crosslinked hybrids and crosslinked hybrids formulated using low-molecular-weight chitosan. Correspondingly, dynamic vapor sorption evidenced significantly lower water vapor sorption for crosslinked scaffolds formulated using high-molecular-weight chitosan.

AB - In this study, synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal, and sorption properties of porous chitosan/gelatin/hyaluronic acid hybrid foams are reported. Fourier transform infrared spectroscopy has been utilized to confirm the covalent attachment of hyaluronic acid to gelatin and chitosan, and covalent chemical crosslinking between gelatin and chitosan. Detailed image analysis of scanning electron microscopy images of the porous scaffold hydrids reveal that the pore size of the materials formulated using either low- or high-molecular-weight chitosan increases significantly upon crosslinking using ethyl(dimethylaminopropyl) carbodiimide/N-Hydroxysuccinimide. These microstructural changes are even more pronounced for the crosslinked hybrid scaffolds formulated using low-molecular-weight chitosan, highlighting a synergistic effect between crosslinking and the use of low-molecular-weight chitosan. Results obtained using differential scanning calorimetry demonstrate a significant reduction in molecular mobility reduction in molecular mobility for crosslinked scaffolds formed using high-molecular-weight chitosan compared to non-crosslinked hybrids and crosslinked hybrids formulated using low-molecular-weight chitosan. Correspondingly, dynamic vapor sorption evidenced significantly lower water vapor sorption for crosslinked scaffolds formulated using high-molecular-weight chitosan.

KW - biopolymers

KW - chitosan

KW - crosslinking

KW - hybrid

KW - molecular weight

KW - scaffold

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U2 - 10.1002/app.44772

DO - 10.1002/app.44772

M3 - Article

AN - SCOPUS:85010689137

SN - 0021-8995

VL - 134

JO - Journal of Applied Polymer Science

JF - Journal of Applied Polymer Science

IS - 18

M1 - 44772

ER -

Synergistic effects of crosslinking and chitosan molecular weight on the microstructure, molecular mobility, thermal and sorption properties of porous chitosan/gelatin/hyaluronic acid scaffolds

Abstract

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Keywords

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