Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation.

D. Busso; D. J. Cohen; V. Da Ros; R. Fissore; P. S. Cuasnicú

Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation.

D. Busso, D. J. Cohen, V. Da Ros, R. Fissore, P. S. Cuasnicú

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.

Original language	American English
Pages (from-to)	407-412
Number of pages	6
Journal	Cellular and molecular biology (Noisy-le-Grand, France)
Volume	49
Issue number	3
State	Published - 1 Jan 2003
Externally published	Yes

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title = "Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation.",

abstract = "Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.",

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AU - Busso, D.

AU - Cohen, D. J.

AU - Da Ros, V.

AU - Fissore, R.

AU - Cuasnicú, P. S.

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AB - Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.

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