Standardization of Animal Models and Techniques for Platelet-Rich Fibrin Production: A Narrative Review and Guideline

Carlos Fernando Mourão*, Adam Lowenstein, Rafael Coutinho Mello-Machado, Shahram Ghanaati, Nelson Pinto, Tomoyuki Kawase, Gutemberg Gomes Alves, Michel Reis Messora

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations


Experimental research is critical for advancing medical knowledge and enhancing patient outcomes, including in vitro and in vivo preclinical assessments. Platelet-rich fibrin (PRF) is a blood by-product that has garnered attention in the medical and dental fields due to its potential for tissue regeneration and wound healing. Animal models, such as rabbits and rats, have been used to produce PRF and examine its properties and applications. PRF has demonstrated potential in the dental and medical fields for reducing inflammation, promoting tissue repair, and accelerating wound healing. This narrative review aims to compare existing evidence and provide guidelines for PRF animal research, emphasizing the importance of standardizing animal models, following ethical considerations, and maintaining transparency and accountability. The authors highlight the necessity to use the correct relative centrifugal force (RCF), standardize centrifugal calibration, and report detailed information about blood collection and centrifuge parameters for reproducible results. Standardizing animal models and techniques is crucial for narrowing the gap between laboratory research and clinical applications, ultimately enhancing the translation of findings from bench to bedside.

Original languageEnglish
Article number482
Issue number4
StatePublished - Apr 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.


  • animal models
  • experimental research
  • guideline
  • platelet-rich fibrin


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