Spironolactone decreases DOCA-salt-induced organ damage by blocking the activation of T helper 17 and the downregulation of regulatory T lymphocytes

Cristián A. Amador, Víctor Barrientos, Juan Peña, Andrés A. Herrada, Magdalena González, Solange Valdés, Loreto Carrasco, Rodrigo Alzamora, Fernando Figueroa, Alexis M. Kalergis, Luis Michea

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

Adaptive immune response has been implicated in inflammation and fibrosis as a result of exposure to mineralocorticoids and a high-salt diet. We hypothesized that in mineralocorticoid-salt-induced hypertension, activation of the mineralocorticoid receptor alters the T-helper 17 lymphocyte (Th17)/regulatory T-lymphocyte/interleukin-17 (IL-17) pathway, contributing to cardiac and renal damage. We studied the inflammatory response and tissue damage in rats treated with deoxycorticosterone acetate and high-salt diet (DOCA-salt), with or without mineralocorticoid receptor inhibition by spironolactone. To determine whether Th17 differentiation in DOCA-salt rats is caused by hypertension per se, DOCA-salt rats received antihypertensive therapy. In addition, to evaluate the pathogenic role of IL-17 in hypertension and tissue damage, we studied the effect of IL-17 blockade with a specific antibody (anti-IL-17). We found activation of Th17 cells and downregulation of forkhead box P3 mRNA in peripheral tissues, heart, and kidneys of DOCA-salt-treated rats. Spironolactone treatment prevented Th17 cell activation and increased numbers of forkhead box P3-positive cells relative to DOCA-salt rats. Antihypertensive therapy did not ameliorate Th17 activation in rats. Treatment of DOCA-salt rats with anti-IL-17 significantly reduced arterial hypertension as well as expression of profibrotic and proinflammatory mediators and collagen deposits in the heart and kidney. We conclude that mineralocorticoid receptor activation alters the Th17/regulatory T-lymphocyte/IL-17 pathway in mineralocorticoid- dependent hypertension as part of an inflammatory mechanism contributing to fibrosis.
Original languageAmerican English
Pages (from-to)797-803
Number of pages7
JournalHypertension
Volume63
Issue number4
DOIs
StatePublished - 1 Jan 2014

Keywords

  • aldosterone
  • fibrosis
  • hypertension
  • inflammation
  • interleukin-17
  • receptors mineralocorticoid

Fingerprint Dive into the research topics of 'Spironolactone decreases DOCA-salt-induced organ damage by blocking the activation of T helper 17 and the downregulation of regulatory T lymphocytes'. Together they form a unique fingerprint.

Cite this