SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene

F. Gil, I. Hernández-Lucas, R. Polanco, N. Pacheco, B. Collao, J. M. Villareal, Gino Nardocci, G. Calva, C. P. Saavedra

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored ompW expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. In silico analyses suggested a putative binding site for the SoxS transcriptional factor at the ompW regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to -54 and ends at about -197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter.

Original languageEnglish
Pages (from-to)2490-2497
Number of pages8
JournalMicrobiology
Volume155
Issue number8
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint Dive into the research topics of 'SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene'. Together they form a unique fingerprint.

Cite this